CAJ | 학술논문

从受石油污染的海洋沉积物中分离得到1株能以柴油为唯一碳源生长的菌株L HOD-2，通过形态特征、生理生化及16S rDNA序列分析，初步鉴定菌株L HOD-2属于埃氏假交替单胞菌（Pseudoalteromonas espejiana）．利用聚氨酯泡沫为载体制备固定化菌，并对其降解特性进行考察．实验结果表明，菌株L HOD-2的最佳生长降解条件为温度25～30℃，p H 7．0～8．0，盐度（体积分数）3％～3．5％，转速150 r/min ．在最佳条件下，LHOD-2对800 mg/L柴油在120 h内的降解率达到85％（质量分数）．聚氨酯泡沫载体具有大孔网状结构，利于菌株生长和传质，固定化菌降解柴油的速率明显高于游离菌．
종수석유오염적해양침적물중분리득도1주능이시유위유일탄원생장적균주L HOD-2，통과형태특정、생리생화급16S rDNA서렬분석，초보감정균주L HOD-2속우애씨가교체단포균（Pseudoalteromonas espejiana）．이용취안지포말위재체제비고정화균，병대기강해특성진행고찰．실험결과표명，균주L HOD-2적최가생장강해조건위온도25～30℃，p H 7．0～8．0，염도（체적분수）3％～3．5％，전속150 r/min ．재최가조건하，LHOD-2대800 mg/L시유재120 h내적강해솔체도85％（질량분수）．취안지포말재체구유대공망상결구，리우균주생장화전질，고정화균강해시유적속솔명현고우유리균．
Strain LHOD-2 was isolated from the petroleum-polluted marine sediments ,which can utilize diesel oil as sole carbon source for grow th .It was identified as Pseudoalteromonas espej iana , according to its morphology and biochemical characteristics ,and 16S rDNA sequence analysis .Then strain LHOD-2 was immobilized on polyurethane foam for investigating the characteristics of immobi-lized cells .The results showed that the optimal conditions for diesel oil degradation by strain LHOD-2 were 25~30 ℃ ,pH of 7 .0~8 .0 ,3% ~3 .5% salinity and rotate velocity of 150 r/min .And there was 85% removal rate within 120 h for 800 mg/L diesel oil under the optimal conditions .It was sug-gested that the macro-reticular structure of polyurethane was beneficial for diesel oil degradation by accelerating the strain grow th and mass transfer .