CAJ | 학술논문

目的探讨低频超声辐射是否增加化学治疗(化疗)药物对人肺癌细胞的凋亡作用.方法将人肺癌A549的细胞分成4组:空白对照组常规培养;单纯使用顺氯氨铂组(单纯化疗组,药物终浓度5mg/L);单纯低频超声辐射处理组(单纯超声组,频率30 kHz,发射强度2.8 W/cm2,作用时间5 min)以及低频超声辐射联合顺氯氨铂化疗组(超声联合化疗组,超声治疗和化疗方法同单纯超声组和单纯化疗组).经过各个条件处理的细胞培养36 h后应用流式细胞仪检测A549细胞凋亡率.结果超声联合化疗组A549细胞凋亡率[(7.84±2.74)％]明显高于单纯化疗组[(5.06±0.88)％]、单纯超声组[(2.71±0.99)％]和空白对照组[(2.64±0.99)％],差异均有统计学意义(P=0.012,P=0.004,P=0.010),同时超声联合化疗组及单纯化疗组A549细胞凋亡率也明显高于空白对照组,差异有统计学意义(P =0.004),单纯超声组与空白对照组间A549细胞凋亡率差异无统计学意义(P =0.055).结论体外低频超声辐射可以增加化疗药物对人肺癌细胞的凋亡作用.
목적 탐토저빈초성복사시부증가화학치료(화료)약물대인폐암세포적조망작용.방법 장인폐암A549적세포분성4조:공백대조조상규배양;단순사용순록안박조(단순화료조,약물종농도5mg/L);단순저빈초성복사처리조(단순초성조,빈솔30 kHz,발사강도2.8 W/cm2,작용시간5 min)이급저빈초성복사연합순록안박화료조(초성연합화료조,초성치료화화료방법동단순초성조화단순화료조).경과각개조건처리적세포배양36 h후응용류식세포의검측A549세포조망솔.결과 초성연합화료조A549세포조망솔[(7.84±2.74)％]명현고우단순화료조[(5.06±0.88)％]、단순초성조[(2.71±0.99)％]화공백대조조[(2.64±0.99)％],차이균유통계학의의(P=0.012,P=0.004,P=0.010),동시초성연합화료조급단순화료조A549세포조망솔야명현고우공백대조조,차이유통계학의의(P =0.004),단순초성조여공백대조조간A549세포조망솔차이무통계학의의(P =0.055).결론 체외저빈초성복사가이증가화료약물대인폐암세포적조망작용.
Objective To investigate whether low intensity ultrasound enhances chemotherapeutic efficacy on human pulmonary carcinoma.Methods The human pulmonary carcinoma cell line A549 cell suspension was divided into four subgroups:routinely cultured A549 cells as control group; A549 cell suspension treated with cisplation group (final concentration was 5 mg/L) ; A549 cell suspension treated with low intensity ultrasound group (30 kHz,2.8 W/cm2,for five minutes) ; A549 cell suspension treated with combination of low intensity ultrasound (30 kHz,2.8 W/cm2,for five minutes) and cisplation group (final concentration was 5 mg/L).The A549 cell suspension treated with various treatments was incubated for 36 h.The A549 cell apoptosis ratio was detected using flow cytometry.Results The A549 apoptosis ratio was significantly higher in the combination of low intensity ultrasound and cisplation (7.84 ± 2.74) ％ than that in the subgroup of cisplation alone (5.06 ± 0.88) ％ (P =0.012) or low intensity ultrasound alone (2.71 ± 0.99) ％ (P =0.004).The apoptosis ratio of the combination of low intensity ultrosound and cisplation,and the ratio of the cisplation alone were all significantly higher than those in the control group (2.64 ±0.99) (P =0.010,0.004) ; there was no significance difference between low intensity ultrasound group and control group (P =0.055).Conclusion Low intensity ultrasound cam enhance the effects of chemotherapeutics on human pulmonary carcinoma cell line A549 in vitro.