CAJ | 학술논문

目的检测马齿苋黄酮提取液中染料木苷的含量。方法用反相高效液相色谱（RP-HPLC）检测马齿苋黄酮部位中染料木苷的含量。色谱柱为SunfireC18column（250.0mm×4.0mm，5.0μm），流动相为甲醇：冰醋酸：水＝45：1：55，流速为0.8ml/min，柱温25℃，柱压2144psi，检测波长为259nm。结果马齿苋黄酮部位中染料木苷与标准品中染料木苷保留时间一致，染料木苷进样量在0.0625～2.0000ng范围内线性关系良好（r＝0.9999），平均回收率98.30％，RSD＝0.57％（n＝5）。结论RP-HPLC测定方法简便，结果可靠，含生药6kg/L的马齿苋黄酮提取液中染料木苷含量为1.66mg/L。
목적검측마치현황동제취액중염료목감적함량。방법용반상고효액상색보（RP-HPLC）검측마치현황동부위중염료목감적함량。색보주위SunfireC18column（250.0mm×4.0mm，5.0μm），류동상위갑순：빙작산：수＝45：1：55，류속위0.8ml/min，주온25℃，주압2144psi，검측파장위259nm。결과마치현황동부위중염료목감여표준품중염료목감보류시간일치，염료목감진양량재0.0625～2.0000ng범위내선성관계량호（r＝0.9999），평균회수솔98.30％，RSD＝0.57％（n＝5）。결론RP-HPLC측정방법간편，결과가고，함생약6kg/L적마치현황동제취액중염료목감함량위1.66mg/L。
Objective To develop reversed phase high-performance liquid chromatography (RP-HPLC)method for the determination of genistin in flavone extract from Portulaca oleracea L. .Methods The chromatographic separation was performed on a Sunfire C18 column(250.0 mm × 4.0 mm ,5.0μm)and eluted with a mobile phase of MeOH: HAC: H2 O(45:1:55).The detection wavelength was set at 259 nm and column temperature was set at 25 ℃.The flow rate was 0.8 ml/min.Results Stable linear relation between the peak area and injected amount ex-isted when the amount was within 0.062 5 ~ 2.000 0 ng for genistin (r= 0.999 9).The average recovery was 98.30% and the RSD was 0.57% (n=5).Conclusion The HPLC method was accurate and sensitive for the deter-mination of genistin in flavone extract from Portulaca oleracea L. .