福建医科大学学报
福建醫科大學學報
복건의과대학학보
JOURNAL OF FUJIAN MEDICAL UNIVERSITY
2013年
3期
133-136
,共4页
周俊香%陈琅%陈巧彬%卢亚琼%曹小妍
週俊香%陳瑯%陳巧彬%盧亞瓊%曹小妍
주준향%진랑%진교빈%로아경%조소연
奥卡西平%癫痫%卡马西平%抗惊厥药%戊四唑%海马%神经元%神经细胞黏附分子类%疾病模型 ,动物
奧卡西平%癲癇%卡馬西平%抗驚厥藥%戊四唑%海馬%神經元%神經細胞黏附分子類%疾病模型 ,動物
오잡서평%전간%잡마서평%항량궐약%무사서%해마%신경원%신경세포점부분자류%질병모형 ,동물
Oxcarbazapine%epilepsy%carbamazepine%anticonvulsants%pentylenetetrazole%hippo-campus%neurons%neural cell adhesion molecules%disease models,animal
目的观察奥卡西平（OXC）对慢性癫痫幼鼠海马神经元中神经细胞黏附分子（NCAM ）表达的影响。方法将50只21日龄SD大鼠随机分为5组：生理盐水（NS）组、戊四氮（PTZ）组、OXC低剂量（100 mg/kg ， LDOXC）组、OXC中剂量（200 mg/kg ，MDOXC）组、OXC高剂量（300 mg/kg ，HDOXC）。建立不同剂量的OXC干预癫痫幼鼠模型。连续用药21 d ，观察幼鼠体质量、行为学表现；免疫组织化学法观察 NCAM 阳性细胞的表达；Real-time PCR检测海马组织中NCAM mRNA的表达。结果 LDOXC、MDOXC、HDOXC组癫痫发作时间延长，发作程度减低（P ＜0．01）；LDOXC、MDOXC、HDOXC组海马组织中NCAM 阳性细胞、NCAM mRNA的表达降低（P＜0．05）。结论 OXC可抑制癫痫幼鼠海马神经元中NCAM 的表达，并存在一定的剂量依赖性。OXC可能通过减少NCAM的表达，抑制PTZ致幼痫鼠海马神经元发生，减轻癫痫引起的脑损伤。
目的觀察奧卡西平（OXC）對慢性癲癇幼鼠海馬神經元中神經細胞黏附分子（NCAM ）錶達的影響。方法將50隻21日齡SD大鼠隨機分為5組：生理鹽水（NS）組、戊四氮（PTZ）組、OXC低劑量（100 mg/kg ， LDOXC）組、OXC中劑量（200 mg/kg ，MDOXC）組、OXC高劑量（300 mg/kg ，HDOXC）。建立不同劑量的OXC榦預癲癇幼鼠模型。連續用藥21 d ，觀察幼鼠體質量、行為學錶現；免疫組織化學法觀察 NCAM 暘性細胞的錶達；Real-time PCR檢測海馬組織中NCAM mRNA的錶達。結果 LDOXC、MDOXC、HDOXC組癲癇髮作時間延長，髮作程度減低（P ＜0．01）；LDOXC、MDOXC、HDOXC組海馬組織中NCAM 暘性細胞、NCAM mRNA的錶達降低（P＜0．05）。結論 OXC可抑製癲癇幼鼠海馬神經元中NCAM 的錶達，併存在一定的劑量依賴性。OXC可能通過減少NCAM的錶達，抑製PTZ緻幼癇鼠海馬神經元髮生，減輕癲癇引起的腦損傷。
목적관찰오잡서평（OXC）대만성전간유서해마신경원중신경세포점부분자（NCAM ）표체적영향。방법장50지21일령SD대서수궤분위5조：생리염수（NS）조、무사담（PTZ）조、OXC저제량（100 mg/kg ， LDOXC）조、OXC중제량（200 mg/kg ，MDOXC）조、OXC고제량（300 mg/kg ，HDOXC）。건립불동제량적OXC간예전간유서모형。련속용약21 d ，관찰유서체질량、행위학표현；면역조직화학법관찰 NCAM 양성세포적표체；Real-time PCR검측해마조직중NCAM mRNA적표체。결과 LDOXC、MDOXC、HDOXC조전간발작시간연장，발작정도감저（P ＜0．01）；LDOXC、MDOXC、HDOXC조해마조직중NCAM 양성세포、NCAM mRNA적표체강저（P＜0．05）。결론 OXC가억제전간유서해마신경원중NCAM 적표체，병존재일정적제량의뢰성。OXC가능통과감소NCAM적표체，억제PTZ치유간서해마신경원발생，감경전간인기적뇌손상。
Objective To explore the effects of oxcarbazapine(OXC)on the expression of NCAM in hippocampus of immature rats with epilepsy . Methods A total of 50 male SD rats around 19 to 21 days of age were randomly allocated into 5 groups :normal saline (NS)group ,pentylenetetrazole (PTZ) group ,low-dose OXC group(100 mg/kg ,LDOXC) ,middle-dose OXC group(200 mg/kg ,MDOXC) and high-dose OXC group(300 mg/kg ,HDOXC) . The animal model of rats with epilepsy treated by different doses of OXC was established . After twenty-one days administration ,changes in behavioral performance were recorded ,and the NCAM protein level and mRNA in hippocampus were examined by immunohisto-chemisty and Real-time PCR respectively . Results Compared with PTZ group ,latent time and degree of epileptic seizures induced by PTZ were prolonged and alleviated in OXC groups (P<0 .01) ,the expression of NCAM in the astrocyte and NCAM mRNA in hippocampus were depressed in OXC groups (P<0 .05) . Conclusion NCAM may correlate with epilepsy closely .OXC could decrease the expression of NCAM with a dosage dependent manner .OXC may alleviate the brain damage caused by epilepsy .
