CAJ | 학술논문

目前常用马铃薯病毒检测的方法，主要包括寄主生物学检测法、抗血清检测法、电子显微镜检测法、酶联免疫检测法和分子生物学检测法。重点讨论了分子生物学方法中的各种RT-PCR方法。同时对各种方法的优缺点进行了比较，并总结出将传统生物学检测技术、免疫学检测技术和分子生物学检测技术相结合必将成为检测植物病毒最经济有效的手段。
목전상용마령서병독검측적방법，주요포괄기주생물학검측법、항혈청검측법、전자현미경검측법、매련면역검측법화분자생물학검측법。중점토론료분자생물학방법중적각충RT-PCR방법。동시대각충방법적우결점진행료비교，병총결출장전통생물학검측기술、면역학검측기술화분자생물학검측기술상결합필장성위검측식물병독최경제유효적수단。
The common test methods for potato virus detection were reviewed, including host biology detection, antiserological detection assay, electron microscopy, enzyme-linked immunosorbent assay and molecular biological detection assay, with emphasis on the various RT-PCR methods of molecular biology methods. At the same time, the merit and demerit were compared with different methods, concluding that the combination of traditional biological detection, immunological detection and molecular biology detection technology would be the most cost-effective means for detection of plant viruses.