CAJ | 학술논문

目的探讨 MMP-9，CD105在胰腺癌发生、发展及转移过程中的意义。方法采用免疫组织化学 S-P 法，检测 MMP-9，CD105在53例胰腺癌组织（实验组）及10例正常胰腺组织（对照组）中的表达，计数微血管密度（MVD），并结合临床病理学资料进行分析比较。结果胰腺癌组织中 MMP-9的阳性表达明显高于正常胰腺组织（P<0.05）。MMP-9的表达与胰腺癌的临床分期及淋巴结有无转移有关（P<0.05）。胰腺癌组织的微血管密度（MVD）明显高于非肿瘤性胰腺组织。CD105的表达水平与胰腺癌临床分期及病理分级有关。在有淋巴结转移的癌组织中，其 MVD 值高于无淋巴结转移组织（P<0.05）。胰腺癌组织中 MMP-9的表达与 MVD 呈正相关（P<0.05）。结论MMP-9的表达与胰腺癌的临床分期和淋巴结转移有关。MVD 值有可能作为判断胰腺癌生物学行为的潜在指标。
목적탐토 MMP-9，CD105재이선암발생、발전급전이과정중적의의。방법채용면역조직화학 S-P 법，검측 MMP-9，CD105재53례이선암조직（실험조）급10례정상이선조직（대조조）중적표체，계수미혈관밀도（MVD），병결합림상병이학자료진행분석비교。결과이선암조직중 MMP-9적양성표체명현고우정상이선조직（P<0.05）。MMP-9적표체여이선암적림상분기급림파결유무전이유관（P<0.05）。이선암조직적미혈관밀도（MVD）명현고우비종류성이선조직。CD105적표체수평여이선암림상분기급병리분급유관。재유림파결전이적암조직중，기 MVD 치고우무림파결전이조직（P<0.05）。이선암조직중 MMP-9적표체여 MVD 정정상관（P<0.05）。결론MMP-9적표체여이선암적림상분기화림파결전이유관。MVD 치유가능작위판단이선암생물학행위적잠재지표。
Objective To explore the significance of MMP-9 and CD105 in oncogenesis, progression and metastasis of pan-creatic carcinoma. Methods Immunohistochemistry methods (S-P) were applied to detect the differential expression of MMP-9, CD105 in Paraffin-embedded tumor specimens of 53 cases pancreatic carcinoma tissue and 10 cases normal pancreatic tissue. The correlations between the expression of MMP-9 and CD105, microvessel density (MVD), and clinicop-athologic features were statistically analyzed with SPSS 12.0. Results The positive expression of MMP-9 was significantly higher in tissues of pancreatic carcinoma than in normal pancreatic tissues (P<0.05). The expression of MMP-9 was correlated with lymph node metastasis and clinical stages (P<0.05). The results of the counted number of MVD in cancer tissues revealed that the MVD of cases with pancreatic carcinoma was significantly higher than that without pancreatic carcinoma (P<0.05). The expression level of CD105 was positively correlated with clinical stages and histological grade (P<0.05). Its MVD was significantly higher in carcinoma tis-sues with lymph node metastasis than in that without lymph node metastasis (P<0.05). There was significantly statistic correlation between the expression of MMP-9 and MVD in pancreatic carcinoma (P<0.05). Conclusions The expression of MMP-9 was significantly correlated with lymph node metastasis and tumor clinical stage. MVD had significant correlation with grade of the disease, indicating its potential value of becoming a new marker to the prognosis evaluation of pancreatic carcinoma.