CAJ | 학술논문

目的分析埃克病毒11型（ Echo11）河南分离株的分子流行病学特征。方法对2010-2012年河南手足口病监测系统中采集的粪便标本，采用RD、Hep2细胞进行病毒分离，对阳性分离物进行VP1区扩增、测序，生物学软件分析测序结果，BLAST比对后确定病毒基因型；扩增10株Echo11河南分离株VP1完整编码区序列，与国内外其他Echo11病毒株进行同源性分析并构建VP1区全基因亲缘进化树，分析其进化来源及不同基因型的流行范围。结果2010-2012年河南手足口病标本共分离出184株非EV71/CA16型肠道病毒，其中Echo1110株，占5．43％。 Echo11河南株VP1区全长共876个核苷酸，编码292个氨基酸；10株Echo11河南株VP1区之间核苷酸同源性为93．1％～100％，氨基酸同源性为97．3％～100％；与原型Gregory株之间核苷酸同源性为77．8％～78．8％，氨基酸同源性为90．8％～91．8％。结论 Echo11也是引起河南省手足口病的主要病原体，河南省存在Echo11病毒A基因型的流行。
목적분석애극병독11형（ Echo11）하남분리주적분자류행병학특정。방법대2010-2012년하남수족구병감측계통중채집적분편표본，채용RD、Hep2세포진행병독분리，대양성분리물진행VP1구확증、측서，생물학연건분석측서결과，BLAST비대후학정병독기인형；확증10주Echo11하남분리주VP1완정편마구서렬，여국내외기타Echo11병독주진행동원성분석병구건VP1구전기인친연진화수，분석기진화래원급불동기인형적류행범위。결과2010-2012년하남수족구병표본공분리출184주비EV71/CA16형장도병독，기중Echo1110주，점5．43％。 Echo11하남주VP1구전장공876개핵감산，편마292개안기산；10주Echo11하남주VP1구지간핵감산동원성위93．1％～100％，안기산동원성위97．3％～100％；여원형Gregory주지간핵감산동원성위77．8％～78．8％，안기산동원성위90．8％～91．8％。결론 Echo11야시인기하남성수족구병적주요병원체，하남성존재Echo11병독A기인형적류행。
Objective To analyze the evolution and genetic characteristics of Echovirus 11 (Echo11) strains isolated from patients with hand food and mouth disease ( HFMD) in Henan province. Methods Enterovirus strains were isolated from stool samples of patients with HFMD from year 2010 to 2012.The sequences of VP1 region of all positive strains were amplified , sequenced and then analyzed by BLSAT to determine their genotypes .The entire VP1 coding regions of 10 Echo11 strains were amplified by RT-PCR, and the homology analysis was conducted among them and other Echo 11 strains published in Gen-Bank.A phylogenetic tree was constructed to evaluate the evolution of Henan isolates and the prevalence of different genotypes .Results From year 2010 to 2012 , 184 non-Enterovirus 71 ( EV71 ) strains and non-Coxsakievirus A16 (CA16) strains causing HFMD were isolated, 10 (5.43%) of with were Echo11 strains. There were 876 nucleotides ( nt) in the complete VP1 gene sequence , encoding 292 amino acids ( aa) .The 10 Echo11 strains shared 93.1%-100% homologies in nt sequences and 97.3%-100% in aa sequences. The homology analysis indicated that Henan strains and prototype strains were highly similar but different with the homology of 77.8%-78.8% in nt and 90.8%-91.8% in aa, respectively.Conclusion Echo11 was one of the pathogens causing HFMD in He-nan province and genotype A was the dominant genotype .