中华普通外科学文献（电子版）
中華普通外科學文獻（電子版）
중화보통외과학문헌（전자판）
CHINESE JOURNAL OF GENERAL SURGERY(ELECTRONIC VERSION)
2013年
4期
278-283
,共6页
陈图锋%华赟鹏%黄婵燕%王钟兴
陳圖鋒%華赟鵬%黃嬋燕%王鐘興
진도봉%화빈붕%황선연%왕종흥
全反式维甲酸%缺血再灌注损伤%炎症%NF-κB%小肠
全反式維甲痠%缺血再灌註損傷%炎癥%NF-κB%小腸
전반식유갑산%결혈재관주손상%염증%NF-κB%소장
All trans retinoic acid%Ischemia reperfusion injury%Inflammation%NF-kappa B%Intestine
目的探讨全反式维甲酸（ATRA）对大鼠小肠缺血再灌注的炎症抑制作用和机制。方法24只雄性SD大鼠，每组8只，随机分为假手术组、阻断组和ATRA组。ATRA组术前以ATRA 15 mg?kg-1?d-1灌胃，阻断组以等体积溶媒二甲基亚砜（DMSO）灌胃，共5d。术中阻断肠系膜上动脉60 min后再灌注120 min，收集各组大鼠末端回肠和血清。光学显微镜下观察回肠病理学改变、行肠黏膜Chiu氏评分；比色法测定血清二胺氧化酶（DAO）含量和回肠组织髓过氧化物酶（MPO）活性；ELISA法测定回肠组织肿瘤坏死因子α（TNF-α）及白介素1β（IL-1β）水平；Western blot检测回肠组织中胞核NF-κB p65蛋白和胞质NF-κB抑制蛋白α（IκBα）的表达量。结果与阻断组相比，ATRA组回肠黏膜病理损伤减轻，Chiu氏评分下降[(2.54±0.69) vs.(3.86±0.83)，P<0.05]，血清DAO含量减少[(18.09±4.21)U/L vs.(24.56±4.92) U/L，P<0.05]，组织TNF-α[(61.37±18.66)pg/g vs.(97.21±24.67) pg/g]、IL-1β水平[(115.24±30.82) pg/g vs.(219.83±54.31)pg/g]和MPO活性[(4.62±1.18) U/g vs.(7.16±1.50) U/g]降低，均P<0.05；胞核NF-κB p65蛋白表达下调[(3.71±0.83) vs.(6.59±1.05)，P<0.05]，胞质IκBα蛋白含量增加[(0.56±0.12) vs.(0.26±0.05)， P<0.05]。结论 ATRA预处理可以抑制NF-κB的激活，减轻组织的过度炎症反应，对大鼠小肠缺血再灌注损伤具有保护作用。
目的探討全反式維甲痠（ATRA）對大鼠小腸缺血再灌註的炎癥抑製作用和機製。方法24隻雄性SD大鼠，每組8隻，隨機分為假手術組、阻斷組和ATRA組。ATRA組術前以ATRA 15 mg?kg-1?d-1灌胃，阻斷組以等體積溶媒二甲基亞砜（DMSO）灌胃，共5d。術中阻斷腸繫膜上動脈60 min後再灌註120 min，收集各組大鼠末耑迴腸和血清。光學顯微鏡下觀察迴腸病理學改變、行腸黏膜Chiu氏評分；比色法測定血清二胺氧化酶（DAO）含量和迴腸組織髓過氧化物酶（MPO）活性；ELISA法測定迴腸組織腫瘤壞死因子α（TNF-α）及白介素1β（IL-1β）水平；Western blot檢測迴腸組織中胞覈NF-κB p65蛋白和胞質NF-κB抑製蛋白α（IκBα）的錶達量。結果與阻斷組相比，ATRA組迴腸黏膜病理損傷減輕，Chiu氏評分下降[(2.54±0.69) vs.(3.86±0.83)，P<0.05]，血清DAO含量減少[(18.09±4.21)U/L vs.(24.56±4.92) U/L，P<0.05]，組織TNF-α[(61.37±18.66)pg/g vs.(97.21±24.67) pg/g]、IL-1β水平[(115.24±30.82) pg/g vs.(219.83±54.31)pg/g]和MPO活性[(4.62±1.18) U/g vs.(7.16±1.50) U/g]降低，均P<0.05；胞覈NF-κB p65蛋白錶達下調[(3.71±0.83) vs.(6.59±1.05)，P<0.05]，胞質IκBα蛋白含量增加[(0.56±0.12) vs.(0.26±0.05)， P<0.05]。結論 ATRA預處理可以抑製NF-κB的激活，減輕組織的過度炎癥反應，對大鼠小腸缺血再灌註損傷具有保護作用。
목적탐토전반식유갑산（ATRA）대대서소장결혈재관주적염증억제작용화궤제。방법24지웅성SD대서，매조8지，수궤분위가수술조、조단조화ATRA조。ATRA조술전이ATRA 15 mg?kg-1?d-1관위，조단조이등체적용매이갑기아풍（DMSO）관위，공5d。술중조단장계막상동맥60 min후재관주120 min，수집각조대서말단회장화혈청。광학현미경하관찰회장병이학개변、행장점막Chiu씨평분；비색법측정혈청이알양화매（DAO）함량화회장조직수과양화물매（MPO）활성；ELISA법측정회장조직종류배사인자α（TNF-α）급백개소1β（IL-1β）수평；Western blot검측회장조직중포핵NF-κB p65단백화포질NF-κB억제단백α（IκBα）적표체량。결과여조단조상비，ATRA조회장점막병리손상감경，Chiu씨평분하강[(2.54±0.69) vs.(3.86±0.83)，P<0.05]，혈청DAO함량감소[(18.09±4.21)U/L vs.(24.56±4.92) U/L，P<0.05]，조직TNF-α[(61.37±18.66)pg/g vs.(97.21±24.67) pg/g]、IL-1β수평[(115.24±30.82) pg/g vs.(219.83±54.31)pg/g]화MPO활성[(4.62±1.18) U/g vs.(7.16±1.50) U/g]강저，균P<0.05；포핵NF-κB p65단백표체하조[(3.71±0.83) vs.(6.59±1.05)，P<0.05]，포질IκBα단백함량증가[(0.56±0.12) vs.(0.26±0.05)， P<0.05]。결론 ATRA예처리가이억제NF-κB적격활，감경조직적과도염증반응，대대서소장결혈재관주손상구유보호작용。
Objective To investigate the anti-inflammatory effect and mechanism of all trans retinoic acid (ATRA) on intestinal ischemia/reperfusion (I/R) injury induced by superior mesenteric artery occlusion (SMAO). Methods Twenty-four Sprague-Dawley male rats were randomly divided into three groups (8 in each group):Sham group, I/R group and ATRA pretreatment group. Rat intestinal I/R models were made by clamping superior mesenteric artery for 60 min. After allowing reperfusion for 120 min, samples of distal ileum and serum were collected. Histological changes and Chiu's scores on the ileum mucosa were evaluated under light microscope. Serum diamine oxidase (DAO) and ileum myeloperoxidase (MPO) activity were analyzed by colorimetry. Ileum tumor necrosis factor (TNF)-αand interleukin(IL)-1βwere detected by enzyme-linked immunosorbent assay method. Ileum protein of nuclear factor kappa B (NF-κB) p65 in nucleus and inhibitor NF-κB-α(IκBα) in cytoplasm was assessed by Western blot. Results Compared with I/R group, ATRA significantly downgraded the mucosal Chiu's scores [(2.54±0.69)vs. (3.86±0.83), P< 0.05], lessened the serum DAO content [(18.09±4.21)U/L vs. (24.56±4.92)U/L, P< 0.05], suppressed the NF-κB p65 expression [(3.71±0.83) vs. (6.59±1.05), P< 0.05] and increased the IκBα expression [(0.56±0.12) vs. (0.26±0.05), P< 0.05] in ileum. Subsequently, the levels of TNF-α and IL-1β or the activity of MPO in ileum were effectively reduced [(61.37±18.66)pg/g vs. (97.21±24.67) pg/g, P<0.05;(115.24±30.82)pg/g vs. (219.83±54.31) pg/g, P<0.05;(4.62±1.18) U/g vs. (7.16±1.50) U/g, P< 0.05; respectively]. Conclusion With the mechanism of inhibiting NF-κB activation, ATRA pretreatment can alleviate the excessive inflammation response which result in attenuating intestinal ischemia/reperfusion injury induced by SMAO in rat.
