CAJ | 학술논문

目的研究升麻总苷提取物（Total Glycoside of Cimicifuga foetida，TGC）的体内、外抗肿瘤活性及其对人肿瘤细胞周期分布的影响。方法 MTT法检测TGC对人肿瘤细胞株的增殖抑制活性；体内实验观察TGC对小鼠肝癌H22移植瘤和人肝癌裸小鼠异种移植瘤Bel-7402的抑制作用；荧光染色法和流式细胞仪检测其对肿瘤细胞周期的影响。结果 TGC可明显抑制人肝癌HepG2细胞的增殖，其IC50值为（76.16±2.94） mg·L-1；TGC 0.8 g·kg-1剂量可明显抑制小鼠肝癌H22和人肝癌Bel-7402裸小鼠移植瘤的生长；TGC 50 mg·L-1可明显诱导人肝癌HepG2细胞阻滞于G2/M 期，呈现出一定的时-效关系。结论 TGC具有明显的体内、外抗肿瘤作用，其活性可能与阻滞肿瘤细胞周期有关。
목적연구승마총감제취물（Total Glycoside of Cimicifuga foetida，TGC）적체내、외항종류활성급기대인종류세포주기분포적영향。방법 MTT법검측TGC대인종류세포주적증식억제활성；체내실험관찰TGC대소서간암H22이식류화인간암라소서이충이식류Bel-7402적억제작용；형광염색법화류식세포의검측기대종류세포주기적영향。결과 TGC가명현억제인간암HepG2세포적증식，기IC50치위（76.16±2.94） mg·L-1；TGC 0.8 g·kg-1제량가명현억제소서간암H22화인간암Bel-7402라소서이식류적생장；TGC 50 mg·L-1가명현유도인간암HepG2세포조체우G2/M 기，정현출일정적시-효관계。결론 TGC구유명현적체내、외항종류작용，기활성가능여조체종류세포주기유관。
Objective To investigate the anti-tumor activity of total glycoside of Cimicifuga foetida L.(TGC) and its mechanism. Methods The growth inhibitory effect of TGC on human hepatoma cell line HepG2 was observed by using assay(MTT);In vivo anti-tumor activity of TGC were evaluated in transplant hepatoma H22 model and human hepatocellular carcinoma Bel-7402 xenograft nude mice model;Cell cycle distribution was assayed by flow cytometry. Results TGC could markedly inhibit the growth of HepG2 (IC50=76.16 ±2.94 mg·L-1). Compared with the solvent group,TGC showed a significant inhibitory effect on H22 and Bel-7402 at a dose of 0.8 g·kg-1. Furthermore,TGC could arrest HepG2 at G2/M phase at a concentration of 50 mg·L-1 in a time-dependent manner. Conclusion TGC had significant anti-tumor effects both in vivo and in vitro,which might be associated with the arresting of the tumor cell cycle at G2/M phase.