CAJ | 학술논문

目的观察参苓白术散对脾虚湿困证溃疡性结肠炎（UC）大鼠结肠菌群的影响，探讨参苓白术散治疗UC的作用机制。方法将40只Wistar大鼠，随机分为正常对照组、脾虚湿困证UC模型组、参苓白术散组、阳性对照药组。采用传统中医学病因与现代医学病因相结合的方法复制UC模型，给药治疗后，提取结肠内容物细菌总DNA，应用肠杆菌基因间重复共有序列基因扩增（ERIC-PCR）技术进行菌群分析。结果4组大鼠的结肠内容物菌丛存在整体差异，模型组ERIC-PCR指纹图谱DNA条带较少，存在6条分布较一致的主带；而其他3组ERIC-PCR指纹图谱DNA条带较多，且主带位置分布无统一趋势，其中参苓白术散组ERIC-PCR指纹图谱与正常组最为接近。结论 UC可能存在较单一的肠道优势菌，参苓白术散对脾虚湿困证UC结肠菌群的失衡具有明显的调整作用，可使肠道优势菌种类增加，恢复菌群平衡状态，从而促进损伤肠组织的恢复。
목적관찰삼령백술산대비허습곤증궤양성결장염（UC）대서결장균군적영향，탐토삼령백술산치료UC적작용궤제。방법장40지Wistar대서，수궤분위정상대조조、비허습곤증UC모형조、삼령백술산조、양성대조약조。채용전통중의학병인여현대의학병인상결합적방법복제UC모형，급약치료후，제취결장내용물세균총DNA，응용장간균기인간중복공유서렬기인확증（ERIC-PCR）기술진행균군분석。결과4조대서적결장내용물균총존재정체차이，모형조ERIC-PCR지문도보DNA조대교소，존재6조분포교일치적주대；이기타3조ERIC-PCR지문도보DNA조대교다，차주대위치분포무통일추세，기중삼령백술산조ERIC-PCR지문도보여정상조최위접근。결론 UC가능존재교단일적장도우세균，삼령백술산대비허습곤증UC결장균군적실형구유명현적조정작용，가사장도우세균충류증가，회복균군평형상태，종이촉진손상장조직적회복。
Objective To observe the influence of Shenlingbaizhusan on intestinal flora of rats modeled by ulcerative colitis (UC)of spleen deficiency and dampness type in order to explore the therapeutic mechanism of Shenlingbaizhusan to UC. Methods 40 healthy Wistar rats were randomly divided into normal group,model group,Shenlingbaizhusan treated group,and sulfasalazine(SASP) group. The damp-heat syndrome of stomach and intestine were copied by multivariate methods which simulated the etiology of Traditional Chinese Medicine. The treatment had been given to the appropriate model. Total bacterial DNA was extracted from the contents of the colon. Flora was analyzed by ERIC-PCR technology. Results There were the differences of the intestinal flora in four groups according to the analysis. The DNA bands of ERIC-PCR fingerprinting electrophoresis were little in model group. The DNA bands of ERIC-PCR fingerprinting electrophoresis were numerous in other three groups. The trend of main-belt location of Shenlingbaizhusan was close to normal group. Conclusion There might be an advantage intestinal bacterium. Shenlingbaizhusan played an important role in adjusting microflora imbalance of UC of spleen deficiency and dampness type,Increasing dominant strain of intestinal,restoring the flora balance status in order to promote the recovery of damaged intestinal tissue.