CAJ | 학술논문

为了更好地理解和应用笔者制定的国家标准《烟叶和烟叶提取物中茄尼醇的测定-高效液相色谱法》，笔者详细说明了该方法的技术要点，筛选出最佳的提取条件和皂化条件。高效液相色谱检测条件为：采用Waters Atlantis T3-C18柱（4.6×150 mm，3μm），柱温35℃；以异丙醇-甲醇（体积比为30∶70）为流动相，流速为1.0 mL/min；采用二极管阵列检测器，检测波长213 nm。实验结果表明，该方法对茄尼醇的检出限为1 mg/kg，在0.1～5.0 g/100g线性范围内，茄尼醇含量与峰面积的相关系数达0.9999以上；方法加标回收率达到95%以上；相对标准偏差小于5%（n=6）。该方法快速、简便、具有良好的精密度和准确性。
위료경호지리해화응용필자제정적국가표준《연협화연협제취물중가니순적측정-고효액상색보법》，필자상세설명료해방법적기술요점，사선출최가적제취조건화조화조건。고효액상색보검측조건위：채용Waters Atlantis T3-C18주（4.6×150 mm，3μm），주온35℃；이이병순-갑순（체적비위30∶70）위류동상，류속위1.0 mL/min；채용이겁관진렬검측기，검측파장213 nm。실험결과표명，해방법대가니순적검출한위1 mg/kg，재0.1～5.0 g/100g선성범위내，가니순함량여봉면적적상관계수체0.9999이상；방법가표회수솔체도95%이상；상대표준편차소우5%（n=6）。해방법쾌속、간편、구유량호적정밀도화준학성。
We elucidate a Chinese standard:Determining solanesolin in tobacco leaves and its extracts using HPLC, so that the users can understand and use this standard more proficiently. The best extraction method and condition of saponification was screen out. The HPLC conditions were as follows: Waters Atlantis T3 C18 column (4.6 mm i.d.×150 mm,3 μm ) was used at 35 ℃ with isopropanol-methanol (30:70, v/v) as the mobile phase with a flow rate of 1.0 mL/min, detector UV-213 nm. The detection limit for tobacco leaves and its extracts was 1 mg/kg. The linear range was from 0.1 to 5.0 g/100g, and correlation coefficients were greater than 0.9999. The recoveries were greater than 95%with relative standard deviations of less than 5%. The method is rapid, simple and accurate.