CAJ | 학술논문

在实验室对采自山东费县费城烟株根部的白色菌丝进行分离、纯化，编号为FBJ，对其致病性、形态特征观察、生物学特性及防治药剂筛选进行了初步研究。结果表明，FBJ对烟草具有致病性，经形态特征及18S rDNA序列分析，FBJ为半知菌亚门，丝孢纲，无孢目，小菌核属的齐整小核菌（Sclerotium rolfsii Sacc.）能引起烟草白绢病。FBJ白绢病菌适合在偏碱性条件下生长；菌丝和菌核致死温度为50℃处理10 min；通过室内药剂筛选，70%代森锰锌可湿性粉剂对FBJ病菌菌丝生长和菌核萌发的抑制效果较好。
재실험실대채자산동비현비성연주근부적백색균사진행분리、순화，편호위FBJ，대기치병성、형태특정관찰、생물학특성급방치약제사선진행료초보연구。결과표명，FBJ대연초구유치병성，경형태특정급18S rDNA서렬분석，FBJ위반지균아문，사포강，무포목，소균핵속적제정소핵균（Sclerotium rolfsii Sacc.）능인기연초백견병。FBJ백견병균괄합재편감성조건하생장；균사화균핵치사온도위50℃처리10 min；통과실내약제사선，70%대삼맹자가습성분제대FBJ병균균사생장화균핵맹발적억제효과교호。
The morphology, biological characteristics, 18SrDNA and fungicide screening of the pathogen isolated from the root of tobacco from Feixian in Shandong province were studied. The results showed that pathogen of FBJ had pathogenicity of tobacco. Through morphological characteristics and 18S rDNA sequence analysis, FBJ was Sclerotium rolfsii Sacc. It could cause southern blight on tobacco. Biological characteristics displayed that the pathogen of FBJ grew well at the pH range of 5.0-10.0, with the optimal pH of 9.0. Hypha and sclerotia viability were completely lost when hyphal agar plugs and sclerotia incubated at 50℃ 10 minutes. Fungicide testing on PDA plates revealed that 70%Mancozeb showed the greatest inhibition of hyphal growth and sclerotia germination of S. rolfsii.