暨南大学学报(自然科学与医学版)
暨南大學學報(自然科學與醫學版)
기남대학학보(자연과학여의학판)
JOURNAL OF JINAN UNIVERSITY(NATURAL SCIENCE & MEDICINE EDITION)
2013年
4期
373-377
,共5页
王旭%徐玲%张帆%徐艳%吴秀丽%杨力建%陈少华%李萡%卢育洪%李扬秋
王旭%徐玲%張帆%徐豔%吳秀麗%楊力建%陳少華%李萡%盧育洪%李颺鞦
왕욱%서령%장범%서염%오수려%양력건%진소화%리萡%로육홍%리양추
多发性骨髓瘤%多发性骨髓瘤(MM)%黏膜相关组织淋巴瘤异位基因1 (MALT1 )%A20%NF-κB%基因表达
多髮性骨髓瘤%多髮性骨髓瘤(MM)%黏膜相關組織淋巴瘤異位基因1 (MALT1 )%A20%NF-κB%基因錶達
다발성골수류%다발성골수류(MM)%점막상관조직림파류이위기인1 (MALT1 )%A20%NF-κB%기인표체
multiple myeloma%MM%MALT1%A20%NF-κB%gene expression
目的:了解多发性骨髓瘤(MM)患者外周血中黏膜相关组织淋巴瘤异位基因1(MALT1)、A20与核转录因子κB(NF-κB)基因的表达特点。方法:采用SYBR GreenⅠ荧光实时定量PCR和相对定量分析法检测20例MM患者(Ⅰ期7例,Ⅱ期3例,Ⅲ期10例)及21例健康人外周血单个核细胞(PBMC)中MALT1、A20和NF-κB基因的表达情况。以β2微球蛋白基因(β2m)作为内参;采用相对定量公式:α-ΔCt ×100%,分别计算MALT1、A20和NF-κB基因的表达水平。结果:MM患者PBMC中的MALT1和A20基因的表达水平较健康人都有明显的降低(P=0.000),而MM中NF-κB基因的表达水平较正常人有所升高,但无统计学差异(P>0.05)。在健康人组,MALT1和NF-κB的表达水平呈现正相关(P=0.001,r=0.666),而在MM组,MALT1、A20与NF-κB 3种基因均不存在明显相关性。同时,Ⅰ、Ⅱ、Ⅲ期MM患者MALT1基因的表达量较健康人明显降低(P<0.05),A20基因的表达量较健康人明显降低(P<0.01),而Ⅰ、Ⅱ、Ⅲ期MM患者MALT1、A20和NF-κB之间的表达情况均无统计学差异。结论:当MALT1-A20介导的细胞免疫和炎症的信号通路发生异常改变时,可能与MM的发生发展有着密切关系。
目的:瞭解多髮性骨髓瘤(MM)患者外週血中黏膜相關組織淋巴瘤異位基因1(MALT1)、A20與覈轉錄因子κB(NF-κB)基因的錶達特點。方法:採用SYBR GreenⅠ熒光實時定量PCR和相對定量分析法檢測20例MM患者(Ⅰ期7例,Ⅱ期3例,Ⅲ期10例)及21例健康人外週血單箇覈細胞(PBMC)中MALT1、A20和NF-κB基因的錶達情況。以β2微毬蛋白基因(β2m)作為內參;採用相對定量公式:α-ΔCt ×100%,分彆計算MALT1、A20和NF-κB基因的錶達水平。結果:MM患者PBMC中的MALT1和A20基因的錶達水平較健康人都有明顯的降低(P=0.000),而MM中NF-κB基因的錶達水平較正常人有所升高,但無統計學差異(P>0.05)。在健康人組,MALT1和NF-κB的錶達水平呈現正相關(P=0.001,r=0.666),而在MM組,MALT1、A20與NF-κB 3種基因均不存在明顯相關性。同時,Ⅰ、Ⅱ、Ⅲ期MM患者MALT1基因的錶達量較健康人明顯降低(P<0.05),A20基因的錶達量較健康人明顯降低(P<0.01),而Ⅰ、Ⅱ、Ⅲ期MM患者MALT1、A20和NF-κB之間的錶達情況均無統計學差異。結論:噹MALT1-A20介導的細胞免疫和炎癥的信號通路髮生異常改變時,可能與MM的髮生髮展有著密切關繫。
목적:료해다발성골수류(MM)환자외주혈중점막상관조직림파류이위기인1(MALT1)、A20여핵전록인자κB(NF-κB)기인적표체특점。방법:채용SYBR GreenⅠ형광실시정량PCR화상대정량분석법검측20례MM환자(Ⅰ기7례,Ⅱ기3례,Ⅲ기10례)급21례건강인외주혈단개핵세포(PBMC)중MALT1、A20화NF-κB기인적표체정황。이β2미구단백기인(β2m)작위내삼;채용상대정량공식:α-ΔCt ×100%,분별계산MALT1、A20화NF-κB기인적표체수평。결과:MM환자PBMC중적MALT1화A20기인적표체수평교건강인도유명현적강저(P=0.000),이MM중NF-κB기인적표체수평교정상인유소승고,단무통계학차이(P>0.05)。재건강인조,MALT1화NF-κB적표체수평정현정상관(P=0.001,r=0.666),이재MM조,MALT1、A20여NF-κB 3충기인균불존재명현상관성。동시,Ⅰ、Ⅱ、Ⅲ기MM환자MALT1기인적표체량교건강인명현강저(P<0.05),A20기인적표체량교건강인명현강저(P<0.01),이Ⅰ、Ⅱ、Ⅲ기MM환자MALT1、A20화NF-κB지간적표체정황균무통계학차이。결론:당MALT1-A20개도적세포면역화염증적신호통로발생이상개변시,가능여MM적발생발전유착밀절관계。
Aim:To understand the expression feature of MALT1 ,A20 and NF-κB in the multiple my-eloma(MM)from peripheral blood mononuclear cell (PBMCs).Methods:Real -time PCR with SYBR Green Ⅰ technique were used to determine the expression of MALT1 ,A20 and NF-κB genes from PBMCs of 20 MM patients(stageⅠ7 samples;stageⅡ3 samples;stage Ⅲ10 samples).The peripheral blood from 21 cases healthy individuals was served as control.By using β2-microglobulin gene (β2m)as an endogenous reference,relative mRNA expression level of MALT1 ,A20 and NF-κB genes was analyzed by using theα-ΔCt ×100%method.Results:The relative mRNA expression level of MALT1 and A20 in PB-MCs from MM patients was significantly lower than those from healthy individuals (P=0.000).Howev-er,the expression level of NF-κB gene was higher than those from healthy individuals (P>0.05 ),but there is no significant difference.In addition,there was a significant positive correlation between the rela-tive expression levels of MALT1 and NF-κB gene.However,there are no significant correlation in MALT1 ,A20 and NF-κB genes.Moreover,the expression level of MALT1 gene was significantly lower than those from healthy individuals (P<0.05 )in stageⅠ,stageⅡ and stage Ⅲ of MM.While the ex-pression level of A20 gene was also significantly lower than those from healthy individuals (P<0.05 ) stageⅠ,stageⅡ and stage Ⅲ of MM.No significant difference expression level of MALT1 ,A20,and NF-κB among stageⅠ.stageⅡ and stage Ⅲ of MM.Conclusion:In MM,when abnormal changes oc-curred in MALT1-A20 mediated cellular immune and inflammatory signaling pathways,it may affect the development of MM.