CAJ | 학술논문

植物在长期进化过程中，形成了一套天然的防御系统。其中植物抗真菌蛋白（antifungal protein，AFP）是主要成员之一，它对多种植物和人体病原真菌生长有抑制作用。本研究以慈菇（Sagittaria sagittifolia）块茎为材料，运用阳离子交换层析和葡聚糖G50凝胶过滤层析技术，结合活性追踪法，对慈菇中的抗真菌蛋白进行了初步分离纯化。SDS-聚丙烯酰胺凝胶电泳鉴定表明，纯化的抗真菌蛋白并不是单一的组分，至少有3个电泳条带，分子量集中在14.4~20.1 kD之间。纯化的抗真菌蛋白对烟草赤星病菌（Alternari alternata）的菌丝生长具有较明显抑制作用。
식물재장기진화과정중，형성료일투천연적방어계통。기중식물항진균단백（antifungal protein，AFP）시주요성원지일，타대다충식물화인체병원진균생장유억제작용。본연구이자고（Sagittaria sagittifolia）괴경위재료，운용양리자교환층석화포취당G50응효과려층석기술，결합활성추종법，대자고중적항진균단백진행료초보분리순화。SDS-취병희선알응효전영감정표명，순화적항진균단백병불시단일적조분，지소유3개전영조대，분자량집중재14.4~20.1 kD지간。순화적항진균단백대연초적성병균（Alternari alternata）적균사생장구유교명현억제작용。
Plant antifungal proteins, the key component of innate immune system in plant, show a great potential in inhibiting or killing various human and plant pathogenic fungi. In this study, we tracked the bioactivity of antifungal proteins, used a series of methods such as cation exchange chromatography and sephadex G50 chromatography to isolate the antifungal proteins from Sagittaria sagittifolia. The results show that the purified antifungal proteins from S. sagittifolia are not a single component, at least three protein bands those molecular weight range from 14.4~20.1 kD were detected by SDS-PAGE. The experiments of antifungal activity show that the purified antifungal proteins apparently inhibit the growth of Alternaria alternata hypha.