井冈山大学学报（自然科学版）
井岡山大學學報（自然科學版）
정강산대학학보（자연과학판）
JOURNAL OF JINGGANGSHAN UNIVERSITY(SCIENCE AND TECHNOLOGY)
2013年
4期
19-27
,共9页
芽短梗霉%黑色素%响应面法%提取
芽短梗黴%黑色素%響應麵法%提取
아단경매%흑색소%향응면법%제취
Aureobasidium pullulans%melanin%response surface methodology%extraction
本研究以出芽短梗霉发酵液为原料提取黑色素，在单因素实验基础上选取实验因素与水平，用二水平实验确定影响黑色素色价的主要因素，然后根据Box-Behnken中心组合实验设计原理采用三因素三水平的响应面分析法，得到最佳提取工艺为：酸沉时间90 min、乙醇添加量93.17%、乙醇浓度90.42%。在此条件下提取黑色素色价可达44.8μ/mL。
本研究以齣芽短梗黴髮酵液為原料提取黑色素，在單因素實驗基礎上選取實驗因素與水平，用二水平實驗確定影響黑色素色價的主要因素，然後根據Box-Behnken中心組閤實驗設計原理採用三因素三水平的響應麵分析法，得到最佳提取工藝為：痠沉時間90 min、乙醇添加量93.17%、乙醇濃度90.42%。在此條件下提取黑色素色價可達44.8μ/mL。
본연구이출아단경매발효액위원료제취흑색소，재단인소실험기출상선취실험인소여수평，용이수평실험학정영향흑색소색개적주요인소，연후근거Box-Behnken중심조합실험설계원리채용삼인소삼수평적향응면분석법，득도최가제취공예위：산침시간90 min、을순첨가량93.17%、을순농도90.42%。재차조건하제취흑색소색개가체44.8μ/mL。
The extraction of melanin from Aureobasidium pullulans fermented liquid was studied in this paper. On the basis of single factor experiments, the main factors that influence the valeur of melanin were determined by two-level experiment, and then the optimum conditions for the extraction of the melanin from A. pullulans fermented liquid were obtained through a three-variable, three-level Box-Benhnken center-united experiment design and response surface methodology. The results showed that the optimum conditions were as follows:acid sinking time 90 min, ethanol add volume 93.17%and ethanol concentration90.42%. Under such conditions, the maximal valeur of melanin was up to 44.8μ/mL.
