CAJ | 학술논문

目的：观察七氟烷预处理对急性缺氧小鼠脑氧化应激的影响，并探讨其与预处理的时间、剂量的关系。方法：60只雄性C57BL/6J小鼠随机分为6组：对照组（Control）、急性缺氧组（H组，6.5％O2，24 h）、2％Sevo 30 min＋H组、2％ Sevo 60 min＋H组、4％ Sevo 30 min＋H组、Propofol＋H组。按试剂盒说明检测脑匀浆丙二醛（MDA）含量及超氧化物岐化酶（SOD）、谷胱甘肽过氧化物酶（GPx）活性。结果：与H组比较，各七氟烷预处理组脑内MDA水平下降，并在2％Sevo 60 min＋H组、4％Sevo 30 min＋H组降低显著，Propofol＋H组则无意义；GPx活性在各七氟烷预处理组有所升高，在4％ Sevo 30 min＋H组上升显著，Propofol＋H组GPx活性下降，但不明显；与预计相反，各预处理使SOD活性显著降低。结论：七氟烷预处理减轻脑氧化应激而具有脑保护作用，并与时间、剂量相关。
목적：관찰칠불완예처리대급성결양소서뇌양화응격적영향，병탐토기여예처리적시간、제량적관계。방법：60지웅성C57BL/6J소서수궤분위6조：대조조（Control）、급성결양조（H조，6.5％O2，24 h）、2％Sevo 30 min＋H조、2％ Sevo 60 min＋H조、4％ Sevo 30 min＋H조、Propofol＋H조。안시제합설명검측뇌균장병이철（MDA）함량급초양화물기화매（SOD）、곡광감태과양화물매（GPx）활성。결과：여H조비교，각칠불완예처리조뇌내MDA수평하강，병재2％Sevo 60 min＋H조、4％Sevo 30 min＋H조강저현저，Propofol＋H조칙무의의；GPx활성재각칠불완예처리조유소승고，재4％ Sevo 30 min＋H조상승현저，Propofol＋H조GPx활성하강，단불명현；여예계상반，각예처리사SOD활성현저강저。결론：칠불완예처리감경뇌양화응격이구유뇌보호작용，병여시간、제량상관。
Aim:To investigate the effect of Sevoflurane(Sevo)preconditioning on oxidative stress of brain in acute hypoxia mice,and the relationship with duration of adminstering drug and concentration. Methods:Sixty male C56BL/6J mice were randomly allocated to 6 groups:Control,Hypoxia(H,6.5%O2 for 24 h),2%Sevo 30 min+H,2%Sevo 60 min+H,4%Sevo 30 min+H,Propofol+H.According to the kit instruction steps,the brain was homogenized for the measurement of malondialdehyde(MDA), superoxide dismutase(SOD),glutathione peroxidase(GPx).Results:Compared with H group,the brain levels of MDA reduced in all Sevo precondition groups,moreover,in the 2%Sevo 60 min+H group and 4% Sevo 30 min+H group,significantly reduced,however Propofol+H group was not statistically signif-icant;the activity of GPx increased in three Sevo precondition groups,and in the 4% Sevo 30 min+H group,this increased significantly,activity of GPx reduced in the Propofol+H group,but has not signifi-cant;the activity of SOD decreased in all precondition groups which was not influenced as expected.Con-clusion:Sevo preconditioning protects the mouse brain against oxidative stress,and the protection is relat-ed to duration of adminstering drug and dose.