CAJ | 학술논문

为探讨大豆球蛋白对小肠内微量元素Ca2+吸收的影响及相关机制。采用自动生化分析仪测定小肠囊内液中Ca2+浓度，并测定小肠囊中段组织中与Ca2+吸收相关的Ca2+-ATP酶和超氧化物歧化酶活性。结果显示，单独应用大豆球蛋白（0.4~1 mg·mL-1）对小肠囊Ca2+吸收无明显影响，但可剂量依赖性抑制小肠粘膜内Ca2+-ATP酶活性，同时剂量依赖性的反馈性诱导增强小肠粘膜内超氧化物歧化酶活性。1 mg·mL-1大豆球蛋白通过抑制Ca2+-ATP酶活性抑制葡萄糖酸钙Ca2+吸收。综上表明，大豆球蛋白通过抑制Ca2+-ATP酶活性阻碍Ca2+的吸收及转运，并可能通过反馈性的增强SOD的活性以减少氧化自由基对粘膜的进一步损伤。
위탐토대두구단백대소장내미량원소Ca2+흡수적영향급상관궤제。채용자동생화분석의측정소장낭내액중Ca2+농도，병측정소장낭중단조직중여Ca2+흡수상관적Ca2+-ATP매화초양화물기화매활성。결과현시，단독응용대두구단백（0.4~1 mg·mL-1）대소장낭Ca2+흡수무명현영향，단가제량의뢰성억제소장점막내Ca2+-ATP매활성，동시제량의뢰성적반궤성유도증강소장점막내초양화물기화매활성。1 mg·mL-1대두구단백통과억제Ca2+-ATP매활성억제포도당산개Ca2+흡수。종상표명，대두구단백통과억제Ca2+-ATP매활성조애Ca2+적흡수급전운，병가능통과반궤성적증강SOD적활성이감소양화자유기대점막적진일보손상。
To evaluate the effects and mechanism of glycinin on Ca2+absorption in small intestine,the Ca2+concentrations of the inner liquid in the everted gut sacs were determined by automatic biochemistry analyzer. Then the homogenized small intestine was applied to evaluate the activities of Ca2+-ATPase and Superoxide Dismutase (SOD). The results showed that single usage of glycinin (0.4~1 mg·mL-1)to everted gut sacs did not significantly change the Ca2+ absorption,whereas with dose-dependent inhibitions of Ca2+-ATPase activities and enhancement of superoxide dismutase activities. But 1 mg·mL -1 glycinin could enhance the Ca2+absorptions with calcium gluconate addition to culture medium. Our work indicated that glycin inhibited Ca2 + absorption and transportation by inhibiting the activity of Ca2+-ATPase,and with feedback mode,the activity of SOD was enhanced to resist the damage of oxygen free radicals to intestinal mucosa.