CAJ | 학술논문

研究黑龙江省奶牛主要肠外致病（奶牛乳房炎、子宫内膜炎）大肠埃希菌的种系分类群。用PCR扩增具有种系分类群标记的chuA、yjaA基因和TSPE4.C2 DNA片段的方法，对黑龙江省84株来源于奶牛乳房炎和41株来源于奶牛子宫内膜炎的大肠埃希菌进行种系进化群分类。奶牛乳房炎所检测菌株中A进化种系群占11.90%。B1种系进化群占88.10%。奶牛子宫内膜炎所检测菌株中A种系进化群占14.63%，B1种系进化亚群占85.37%。未检测到属于B2和D群的肠外强毒力株。大肠杆菌分离株接种小鼠剖检可见明显病变。通过遗传进化分析，引起黑龙江奶牛肠外致病大肠埃希菌主要属于B1种系进化群，为奶牛乳房炎和子宫内膜炎疫苗的研制提供理论依据。
연구흑룡강성내우주요장외치병（내우유방염、자궁내막염）대장애희균적충계분류군。용PCR확증구유충계분류군표기적chuA、yjaA기인화TSPE4.C2 DNA편단적방법，대흑룡강성84주래원우내우유방염화41주래원우내우자궁내막염적대장애희균진행충계진화군분류。내우유방염소검측균주중A진화충계군점11.90%。B1충계진화군점88.10%。내우자궁내막염소검측균주중A충계진화군점14.63%，B1충계진화아군점85.37%。미검측도속우B2화D군적장외강독력주。대장간균분리주접충소서부검가견명현병변。통과유전진화분석，인기흑룡강내우장외치병대장애희균주요속우B1충계진화군，위내우유방염화자궁내막염역묘적연제제공이론의거。
Researched distribution of phylogenetic groups among extraintestinal pathogenic Escherichia coli isolates from bovine mastitis,Bovine endometritis in Heilongjiang. 84 E.coli isdates from bovine mastitis and 41 E.coli isdates from Bovine endometritis in Heilongjiang were PCR amplified for genetic markers of chuA,yjaA and TSPE4.C2. The result showed that 11.90% E.coli isolates belonged to group A and 88.10%to group B1 from bovine mastitis,14.63%E.coli isolates belonged to group A and 85.37%to group B1 from Bovine endometritis,no-one belonged to B2 and D groups. Obviously pathological changes were observed in internal organs of dissected experiment mouse. Extraintestinal pathogenic E.coli isolates from bovine for the predominant Phylogenetic group was group B1,this research provided a theoretical basis to prepare bovine mastitis and endometritis vaccine.