CAJ | 학술논문

采用RT-PCR的方法，根据栽培大豆的RNF12基因全长设计特异引物，从野生大豆克隆到GsRNF12基因。序列分析表明，该基因的723 bp的开放阅读框（ORF），编码241个氨基酸。序列分析结果表明：野大豆GsRNF12基因的氨基酸序列与大豆、苜蓿同源性>80%，并构建了GsRNF12基因的表达载体。
채용RT-PCR적방법，근거재배대두적RNF12기인전장설계특이인물，종야생대두극륭도GsRNF12기인。서렬분석표명，해기인적723 bp적개방열독광（ORF），편마241개안기산。서렬분석결과표명：야대두GsRNF12기인적안기산서렬여대두、목숙동원성>80%，병구건료GsRNF12기인적표체재체。
The GsRNF12 gene from Glycine soja was amplified by RT-PCR. The result showed that the full length cDNA of Glycine soja gene 723 bp and contained a open reading frame (ORF)encoding a polypeptide of 241 b amino acid.The GsRNF12 proteins from Glycine max L Medicago truncatula showed more than 80% identities based on amino acid sequences alignment. The plant expression vector Pegad-GsRNF12 was cons tructed.