河北北方学院学报（自然科学版）
河北北方學院學報（自然科學版）
하북북방학원학보（자연과학판）
JOURNAL OF HEBEI NORTH UNIVERSITY(NATURAL SCIENCE EDITION)
2013年
4期
12-14,20
,共4页
赵恒成%穆红光%陈丽肖%甄攀
趙恆成%穆紅光%陳麗肖%甄攀
조항성%목홍광%진려초%견반
瑞香狼毒%总酚酸%没食子酸%分光光度法
瑞香狼毒%總酚痠%沒食子痠%分光光度法
서향랑독%총분산%몰식자산%분광광도법
Stellera chamaejasme%total phenolic acid%gallic acid%spectrophotometry
目的建立瑞香狼毒中总酚酸含量测定方法，并比较不同溶剂的提取效率。方法乙醚脱脂，70％乙醇提取；没食子酸为对照品；分光光度法测定总酚酸含量。结果标准曲线为 A＝1.8316C-0.0505， r＝0.9990，没食子酸含量在0.095～0.47μg·ml-1范围内呈良好线性关系，精密度RSD为1.6％，稳定性RSD为3.8％，回收率为99.47％。瑞香狼毒生药中总酚酸的含量为3.29 mg·g-1，提取物中总酚酸的含量为3.26 mg·ml-1。结论70％乙醇提取效率最高。测定方法简便、快速、灵敏、准确，可作为瑞香狼毒中总酚酸含量的测定方法。
目的建立瑞香狼毒中總酚痠含量測定方法，併比較不同溶劑的提取效率。方法乙醚脫脂，70％乙醇提取；沒食子痠為對照品；分光光度法測定總酚痠含量。結果標準麯線為 A＝1.8316C-0.0505， r＝0.9990，沒食子痠含量在0.095～0.47μg·ml-1範圍內呈良好線性關繫，精密度RSD為1.6％，穩定性RSD為3.8％，迴收率為99.47％。瑞香狼毒生藥中總酚痠的含量為3.29 mg·g-1，提取物中總酚痠的含量為3.26 mg·ml-1。結論70％乙醇提取效率最高。測定方法簡便、快速、靈敏、準確，可作為瑞香狼毒中總酚痠含量的測定方法。
목적건립서향랑독중총분산함량측정방법，병비교불동용제적제취효솔。방법을미탈지，70％을순제취；몰식자산위대조품；분광광도법측정총분산함량。결과표준곡선위 A＝1.8316C-0.0505， r＝0.9990，몰식자산함량재0.095～0.47μg·ml-1범위내정량호선성관계，정밀도RSD위1.6％，은정성RSD위3.8％，회수솔위99.47％。서향랑독생약중총분산적함량위3.29 mg·g-1，제취물중총분산적함량위3.26 mg·ml-1。결론70％을순제취효솔최고。측정방법간편、쾌속、령민、준학，가작위서향랑독중총분산함량적측정방법。
Objective To establish a method for content determination of total phenolic acid in Stellera chamaejasme,and to compare the extraction efficiencies of different solvents.Method Ether was used to degrease,70% ethanol was used to extract, spectrophotometry was to determine, and gallic acid was taken as the reference substance.Resutlt The standard curve was A=1.8316C-0.0505,r=0.9990,and the linear range was 0.095-0.47μg/ml.The RSD of the precision was 1.6%.The RSD of the stability was 3.8%.The mean recovery rate was 99.47%.The contents of total phenolic acid in Stellerachamae-jasme and the extractive of 70% ethanol were 3.29 mg/g and 3.26 mg/ml,respectively.Conclusion The best solvent was 70% ethanol.This method is simple,accurate,sensitive and suitable for determining the total phenolic acid in Sterllera chamaejasme.
