CAJ | 학술논문

为获取更多红色素,进行了对液态发酵的最佳营养液组成和环境条件研究。最佳碳源和氮源确定为葡萄糖和谷氨酸钠,添加浓度分别是30 g/L和1.5 g/L时,最适宜产红曲色素。研究的三种微量元素中,Fe2+明显有利于提高红色素产量,Mn2+也有提高色素产量的作用,但不如 Fe2+添加效果好。最适宜培养基起始 pH 值为6.5,摇瓶旋转速度为700 rpm。在最佳条件下,分批发酵时生物体产量为0.20 gDCW/g葡萄糖,红色素产量为32.5OD500g/(DCW.h)。
위획취경다홍색소,진행료대액태발효적최가영양액조성화배경조건연구。최가탄원화담원학정위포도당화곡안산납,첨가농도분별시30 g/L화1.5 g/L시,최괄의산홍곡색소。연구적삼충미량원소중,Fe2+명현유리우제고홍색소산량,Mn2+야유제고색소산량적작용,단불여 Fe2+첨가효과호。최괄의배양기기시 pH 치위6.5,요병선전속도위700 rpm。재최가조건하,분비발효시생물체산량위0.20 gDCW/g포도당,홍색소산량위32.5OD500g/(DCW.h)。
For the purpose of mass producing Monascus red pigments,optimum medium composition and environmental conditions were investigated in submerged flask cultures.The optimum carbon and nitrogen sources were determined to be 30 g/L of glucose and 1.5 g/L of monosodium glutamate (MSG).Of the three metals examined,Fe2+ showed the strongest stimulatory effect on pigment pro-duction of some stimulatory effect was also found in Mn2+.Optimum pH and agitation speed were de-termined to be 6.5 and 700 rpm.Under the optimum culture conditions batch fermentation showed that the maximum biomass yield and specific productivity of red pigments were 0.20 gDCW/g glucose and 32.5OD500.gDCW-1 .h-1 ,respectively.