CAJ | 학술논문

根据已获得的广藿香转录组数据中的PSY转录本序列,利用Primer 3在线设计基因全长扩增引物,采用RT-PCR方法获得广藿香的八氢番茄红素合成酶(phytoene synthase,PSY)基因。并利用在线分析平台和生物软件对该其因进行生物信息学分析。获得的广藿香PSY1基因长1550 bp,编码439个氨基酸,命名为PcPSY1,GenBank登录号为KC862310；预测了PcPSY1编码蛋白的结构与功能,且基于PSY基因利用 NJ法构建了与21个不同物种间的进化树,进化树表明广藿香PcPSY 1基因与桂花的PSY序列亲缘关系最近。成功克隆并分析广藿香PcPSY 1基因的全长序列,为进一步阐明广藿香萜类代谢途径奠定基础。
근거이획득적엄곽향전록조수거중적PSY전록본서렬,이용Primer 3재선설계기인전장확증인물,채용RT-PCR방법획득엄곽향적팔경번가홍소합성매(phytoene synthase,PSY)기인。병이용재선분석평태화생물연건대해기인진행생물신식학분석。획득적엄곽향PSY1기인장1550 bp,편마439개안기산,명명위PcPSY1,GenBank등록호위KC862310；예측료PcPSY1편마단백적결구여공능,차기우PSY기인이용 NJ법구건료여21개불동물충간적진화수,진화수표명엄곽향PcPSY 1기인여계화적PSY서렬친연관계최근。성공극륭병분석엄곽향PcPSY 1기인적전장서렬,위진일보천명엄곽향첩류대사도경전정기출。
Phytoene synthase (PSY)is a key enzyme in plant terpenoid biosynthetic pathway.According the tran-scriptome dataset of Pogostmen cablin,one unique sequence encoding PSY was discovered.The primers were de-signed according to the transcript sequence of PcPSY1 from the P .cablin transcriptome dataset with Primer 3 on-line.The open reading frame of PcPSY1 was cloned using RT-PCR strategy.The cDNA named as PcPSY1 contains a 1 550 bp open reading frame and encodes a predicted protein of 439 amino acids.The GenBank accession number for this gene is KC862310.PcPSY1 has no signal peptide.PcPSY1 is highly homologous to other PSY protein in 21 dif-ferent species and is more related to that from Osmanthus fragrans.This study cloned and analyzed mechanism of terpenoid biosynthesis in Pogostemon cablin plants.