CAJ | 학술논문

以红麻细胞质雄性不育系L23A及其保持系L23B为材料,比较其花药淀粉及可溶性糖含量变化并分析呼吸速率、活性氧产生速率、丙二醛(MDA)含量以及活性氧清除酶(POD、SOD)含量变化,来探讨活性氧伤害与红麻雄性不育的关系。结果表明：在小孢子发育的单核期,不育系呼吸速率与保持系差异不明显,但不育系花药O-2?含量高于保持系；在双核期,不育系的呼吸速率明显低于其保持系,但不育系花药O-2?含量与保持系花药相近；不育系在单核期和双核期的呼吸速率几乎没有变化,而保持系同一时期的呼吸速率呈明显增高趋势；在不育系败育过程中,药隔维管组织中的大颗粒淀粉含量几乎不变,且不育系花药中的可溶性糖含量在单核期和双核期均低于保持系。推测是由于不育系花药中抗氰呼吸降低,一方面导致花药物质代谢和能量代谢的紊乱,不育系花药不能利用药隔组织中的淀粉粒,另一方面不能有效将细胞内过多电子通过抗氰呼吸传至 O2,引致不育花药中O-2?升高,从而导致MDA含量在单核期和双核期均高于保持系,同时POD的活性在单核期及双核期均低于保持系,而SOD活性在单核期高于保持系,在双核期则低于保持系。不育系花药在发育中,花药O-2?和MDA过量积累,以及SOD和POD酶活性降低,导致活性氧产生与清除失去平衡,花粉败育。
이홍마세포질웅성불육계L23A급기보지계L23B위재료,비교기화약정분급가용성당함량변화병분석호흡속솔、활성양산생속솔、병이철(MDA)함량이급활성양청제매(POD、SOD)함량변화,래탐토활성양상해여홍마웅성불육적관계。결과표명：재소포자발육적단핵기,불육계호흡속솔여보지계차이불명현,단불육계화약O-2?함량고우보지계；재쌍핵기,불육계적호흡속솔명현저우기보지계,단불육계화약O-2?함량여보지계화약상근；불육계재단핵기화쌍핵기적호흡속솔궤호몰유변화,이보지계동일시기적호흡속솔정명현증고추세；재불육계패육과정중,약격유관조직중적대과립정분함량궤호불변,차불육계화약중적가용성당함량재단핵기화쌍핵기균저우보지계。추측시유우불육계화약중항청호흡강저,일방면도치화약물질대사화능량대사적문란,불육계화약불능이용약격조직중적정분립,령일방면불능유효장세포내과다전자통과항청호흡전지 O2,인치불육화약중O-2?승고,종이도치MDA함량재단핵기화쌍핵기균고우보지계,동시POD적활성재단핵기급쌍핵기균저우보지계,이SOD활성재단핵기고우보지계,재쌍핵기칙저우보지계。불육계화약재발육중,화약O-2?화MDA과량적루,이급SOD화POD매활성강저,도치활성양산생여청제실거평형,화분패육。
Kenaf (Hibiscus cannabinus)anther of the cytoplasmic male-sterile(CMS)line L23A and its maintainer line L23B were employeded as experimental materials to reveal the relationship between CMS and metabolisms of re-active oxygen species (ROS)by observing changes of starch and soluble sugar contents and comparing productions of superoxide anion radical(O-2?),respiratory rate as well as content of malonadehyde (MDA)and active oxygen ex-terminating enzymes (SOD and POD).The results showed that there was no evident difference between respiratory rate of CMS line and that of maintainer line at mononuclear stage,but anther of CMS line had higher content of O-2?than that of maintainer line at this stage.Respiratory rate in anther of the CMS was lower than that of maintainer line at binucleate pollen stage,while O-2? content in sterile anther approximated to that in fertile anther of maintainer line.There was no evident change in respiratory rate of CMS line at mononuclear stage and binucleate pollen stage, but respiratory rate in anther of maintainer line obviously increased from mononuclear stage to binucleate pollen stage.With the growth of anther,big starch grains content in L23A connective did not change during its microsporo-genesis and soluble sugar content in L23A was lower than that of L23B at both of mononuclear stage and binucleate pollen stage.This possibility gave rise to the speculations that the decline of cyanide-resistant respiration in anther lead to disturbance of material metabolism and energy metabolism and starch grains presented in the connective tissue were not utilized by anther of the CMS line,meanwhile the intracellular surplus electrons were not effectively passed on to O2 by cyanideGresistant respiration,which leads to increase O-2? content in anther of CMS line so that there is higher MDA content in anther of CMS line at mononuclear stage and binucleate pollen stage.Simultaneously,POD activity was lower than that of its maintainer line at mononuclear stage and binucleate pollen stage.While activitiy of SOD was higher than that of its maintainer line at mononuclear stage,but lower than that of its maintainer line at bi-nucleate pollen stage.During the abortion of CMS anther,O-2?and MDA excessively accumulate in CMS anther and activity of SOD and POD would decline,which disrupted the balance between the production and elimination of ROS and made pollen mother cells apoptosis.