CAJ | 학술논문

研究过氧化氢内源消除剂和交替氧化酶专一性抑制剂影响渗透胁迫对水稻根系的伤害。结果表明：PEG 6000胁迫抑制了水稻幼根的生长,降低了相对含水量、增加了 H2 O2含量,并导致细胞死亡。用5 mmol?L-1二甲基硫脲(过氧化氢内源消除剂,dimethylthiourea,DMTU)预处理水稻幼根能明显降低PEG胁迫下水稻幼根过氧化氢的含量,缓解细胞死亡和相对含水量的降低,但对水稻根的生长影响较小。在 PEG胁迫下,用1 mmol?L-1水杨基氧肟酸(交替氧化酶专一性抑制剂,salicylhydroxamic acid,SHAM)预处理水稻幼根能显著降低水稻幼根的生长和相对含水量,并增加水稻幼根的过氧化氢含量和细胞的死亡程度。这说明 DMTU能缓解PEG胁迫对水稻根系伤害,而SHAM加剧了PEG胁迫对水稻根系伤害。
연구과양화경내원소제제화교체양화매전일성억제제영향삼투협박대수도근계적상해。결과표명：PEG 6000협박억제료수도유근적생장,강저료상대함수량、증가료 H2 O2함량,병도치세포사망。용5 mmol?L-1이갑기류뇨(과양화경내원소제제,dimethylthiourea,DMTU)예처리수도유근능명현강저PEG협박하수도유근과양화경적함량,완해세포사망화상대함수량적강저,단대수도근적생장영향교소。재 PEG협박하,용1 mmol?L-1수양기양우산(교체양화매전일성억제제,salicylhydroxamic acid,SHAM)예처리수도유근능현저강저수도유근적생장화상대함수량,병증가수도유근적과양화경함량화세포적사망정도。저설명 DMTU능완해PEG협박대수도근계상해,이SHAM가극료PEG협박대수도근계상해。
The effects of the scavenger of H2 O2 and specific inhibitor of alternative oxidase on the osmotic stress-in-duced damages to rice roots were studied.The results showed that PEG 6000 inhibited the growth of rice roots,de-creased the relative water content,inceased the H2 O2 content,and caused cell death.Pretreatment with 5 mmol?L-1 dimethylthiourea(DMTU),a scavenger of H2 O2 ,significantly decreased the increment in the H2 O2 content and par-tially alleviated the root cell death and the decrease of the relative water content induced by PEG 6000.However, DMTU had little effect on the growth of rice roots exposed to PEG stress.Under the PEG-induced stress,pretreat-ment with 1 mmol?L-1 salicylhydroxamic acid(SHAM),a specific inhibitor of alternative oxidase,significantly de-creased the growth of rice roots and relative water content,but increased the levels of H2 O2 content and cell death. The results suggested that DMTU could alleviate the osmotic stressGinduced damages to rice roots,whereas SHAM could intensify those damages.