中国水产科学
中國水產科學
중국수산과학
Journal of Fishery Sciences of China
2013年
5期
990-996
,共7页
王磊%陈松林%张英平%范彩霞%刘洋%田永胜%邓寒%刘寿堂%孙德强%谢明树%李文龙
王磊%陳鬆林%張英平%範綵霞%劉洋%田永勝%鄧寒%劉壽堂%孫德彊%謝明樹%李文龍
왕뢰%진송림%장영평%범채하%류양%전영성%산한%류수당%손덕강%사명수%리문룡
牙鲆%家系%F2%F3%雌核发育%鳗弧菌%抗病力
牙鲆%傢繫%F2%F3%雌覈髮育%鰻弧菌%抗病力
아평%가계%F2%F3%자핵발육%만호균%항병력
Paralichthys olivaceus%family%F2 generation%F3 generation%gynogenesis%Vibrio anguillarum%dis-ease resistance
为了培育牙鲆(Paralichthys olivaceus)抗鳗弧菌(Vibrio anguillarum)病的品系或品种,2009和2012年利用中国牙鲆抗病群体、从日本和韩国引进的牙鲆群体以及2007年和2009年从大量牙鲆家系中选留的优良家系为亲本,通过巢式杂交、三元杂交及雌核发育等方法,分别于2009年和2012年建成牙鲆家系43个和65个,选取2009年的33个家系和2012年的43个家系进行鳗弧菌感染实验,共筛选出13个抗病家系,其中3个家系的存活率极显著高于对照组(P<0.01),另外10个家系的存活率显著高于对照组(P<0.05),这13个家系包含F3家系、雌核发育一代和二代家系各1个, F2家系3个,在以上6个家系中，除了1个F2家系,其他家系的亲本均来自于抗病家系且鳗弧菌感染存活率的变异系数都低于10%。对连续三代抗病家系进行分析,发现在上述13个抗病家系和2007年筛选出的3个抗病家系共16个抗病家系中有13个家系来自于中国牙鲆抗病群体相关,结果表明,部分F1、F2、F3和雌核发育家系较好地遗传了其亲本的抗病性能,抗病性能稳定,为培育抗鳗弧菌病的牙鲆品系或品种奠定了基础。
為瞭培育牙鲆(Paralichthys olivaceus)抗鰻弧菌(Vibrio anguillarum)病的品繫或品種,2009和2012年利用中國牙鲆抗病群體、從日本和韓國引進的牙鲆群體以及2007年和2009年從大量牙鲆傢繫中選留的優良傢繫為親本,通過巢式雜交、三元雜交及雌覈髮育等方法,分彆于2009年和2012年建成牙鲆傢繫43箇和65箇,選取2009年的33箇傢繫和2012年的43箇傢繫進行鰻弧菌感染實驗,共篩選齣13箇抗病傢繫,其中3箇傢繫的存活率極顯著高于對照組(P<0.01),另外10箇傢繫的存活率顯著高于對照組(P<0.05),這13箇傢繫包含F3傢繫、雌覈髮育一代和二代傢繫各1箇, F2傢繫3箇,在以上6箇傢繫中，除瞭1箇F2傢繫,其他傢繫的親本均來自于抗病傢繫且鰻弧菌感染存活率的變異繫數都低于10%。對連續三代抗病傢繫進行分析,髮現在上述13箇抗病傢繫和2007年篩選齣的3箇抗病傢繫共16箇抗病傢繫中有13箇傢繫來自于中國牙鲆抗病群體相關,結果錶明,部分F1、F2、F3和雌覈髮育傢繫較好地遺傳瞭其親本的抗病性能,抗病性能穩定,為培育抗鰻弧菌病的牙鲆品繫或品種奠定瞭基礎。
위료배육아평(Paralichthys olivaceus)항만호균(Vibrio anguillarum)병적품계혹품충,2009화2012년이용중국아평항병군체、종일본화한국인진적아평군체이급2007년화2009년종대량아평가계중선류적우량가계위친본,통과소식잡교、삼원잡교급자핵발육등방법,분별우2009년화2012년건성아평가계43개화65개,선취2009년적33개가계화2012년적43개가계진행만호균감염실험,공사선출13개항병가계,기중3개가계적존활솔겁현저고우대조조(P<0.01),령외10개가계적존활솔현저고우대조조(P<0.05),저13개가계포함F3가계、자핵발육일대화이대가계각1개, F2가계3개,재이상6개가계중，제료1개F2가계,기타가계적친본균래자우항병가계차만호균감염존활솔적변이계수도저우10%。대련속삼대항병가계진행분석,발현재상술13개항병가계화2007년사선출적3개항병가계공16개항병가계중유13개가계래자우중국아평항병군체상관,결과표명,부분F1、F2、F3화자핵발육가계교호지유전료기친본적항병성능,항병성능은정,위배육항만호균병적아평품계혹품충전정료기출。
Japanese flounder(Paralichthys olivaceus) is a widely cultured marine fish species in China and is highly valued because of its fast growth and good flavor. However, frequent occurrence of disease and subsequent losses limit profitability and aquaculture development. Breeding a new flounder strain with enhanced disease re-sistance is an important task in marine fish aquaculture. In the present study, new Japanese flounder families were established based on Japanese stock (JS), Korean stock (KS), and stock with high resistance to Vibrio anguillarum (RS) by nested hybrid, cross, and gynogenesis. Forty-three and sixty-five Japanese flounder families were estab-lished in 2009 and 2012, respectively. Disease resistance was investigated using 33 families in 2009 and 43 in 2012 through intraperitoneal injection challenged with V. anguillarum. The results demonstrate that different families exhibited variable survival rates and 13 families with high disease resistance were obtained. All 13 fami-lies exhibited significantly higher survival rates compared with the control, however, some were more significant than others (P<0.01 in 3 families; P<0.05 in 10 families). Of these 13 families, three were derived from the F2 family, one from the F3 family, and two belonged to the first and second gynogenesis generations(G1 and G2). In these six families, disease resistance was high in the parents, with the exception of the F2 family; their survival rate variation coefficients were<10%. Analysis of disease resistance in the three successive generations revealed that, 13 of the 16 disease-resistance families which included 13 families mentioned above and 3 resistant families sereened in 2007 were derived from RS. In conclusion, some F2, F3, G1, and G2 families exhibited enhanced dis-ease resistance inherited from their parents, indicating that the establishment of a disease-resistant strain based on these families is possible.
