CAJ | 학술논문

本研究采用石蜡连续切片技术,对出膜后0~64 d 的达氏鲟(Acipenser dabryanus)视网膜结构、视觉特性及相关机能进行研究。结果显示,达氏鲟初孵仔鱼的视网膜没有分化,视单锥细胞在36 h 出现,视杆细胞在6 d 时出现,7 d 时视网膜各层分化完毕。最小分辨角由8 d 的13.26′下降到64 d 的3.37′,仔鱼发育到13 d 时表现出明显的视网膜运动反应。随着仔鱼发育,视单锥细胞及神经节细胞密度不断降低,视杆细胞密度不断增加,外核层细胞核与视锥细胞及神经节细胞数量的比值均不断增大。结果表明6~12 d 是达氏鲟视网膜结构快速发育和视觉特性发生明显变化的时期,这种变化与其生态变迁、趋光性及摄食方式的变化相适应。本研究旨在探明达氏鲟视网膜的发育特性及其相关机能特点,为开展规模化繁育与种群资源的保护提供理论依据。
본연구채용석사련속절편기술,대출막후0~64 d 적체씨심(Acipenser dabryanus)시망막결구、시각특성급상관궤능진행연구。결과현시,체씨심초부자어적시망막몰유분화,시단추세포재36 h 출현,시간세포재6 d 시출현,7 d 시시망막각층분화완필。최소분변각유8 d 적13.26′하강도64 d 적3.37′,자어발육도13 d 시표현출명현적시망막운동반응。수착자어발육,시단추세포급신경절세포밀도불단강저,시간세포밀도불단증가,외핵층세포핵여시추세포급신경절세포수량적비치균불단증대。결과표명6~12 d 시체씨심시망막결구쾌속발육화시각특성발생명현변화적시기,저충변화여기생태변천、추광성급섭식방식적변화상괄응。본연구지재탐명체씨심시망막적발육특성급기상관궤능특점,위개전규모화번육여충군자원적보호제공이론의거。
We studied the retina structure, visual characteristics, and correlated function of Dabry’s sturgeon (Acipenser dabryanus) ranging from newly hatched larvae to 64-day-old juveniles using paraffin serial sections. The main results are as follows: Larvae exhibited undifferentiated retina at hatching. Single cones appeared after 36 hours and rods appeared after 6 days. After 7 days, the visual system had completely differentiated. The ap-parent retinomotor responses first occurred on day 13. However, the minimum separable angle (α) decreased from 13.26′on day 8 to 3.37′on day 64. During the developmental period, the density of single cones and ganglion cells decreased while that of the rods correspondingly increased. The ratio of nuclei of the outer nuclear layer to gan-glion cells per 100μm increased, as did the ratio of nuclei of the outer nuclear layer to single cones. Our investi-gation revealed that the retina structure and visual characteristics changed rapidly from day 6 to 12 to adapt to ecological shifts and changes in photo taxis and feeding behavior.