中国水产科学
中國水產科學
중국수산과학
Journal of Fishery Sciences of China
2013年
5期
911-917
,共7页
李广丽%邓思平%孙晶%王文达%师尚丽%朱春华
李廣麗%鄧思平%孫晶%王文達%師尚麗%硃春華
리엄려%산사평%손정%왕문체%사상려%주춘화
胡子鲇%性分化%芳香化酶抑制剂%Cyp19a1b%Foxl2
鬍子鲇%性分化%芳香化酶抑製劑%Cyp19a1b%Foxl2
호자점%성분화%방향화매억제제%Cyp19a1b%Foxl2
Clarias fuscus%sex differentiation%aromatase inhibitor%Foxl2%Cyp19a1b
采用组织学和荧光实时定量PCR方法,检测饲喂不同剂量芳香化酶抑制剂来曲唑(letrozole,50、100和200μg/g)对胡子鲇(Clarias fuscus)性腺组织学和性别比率的影响,以及200μg/g 来曲唑对性分化前后脑型芳香化酶基因(Cyp19a1b)和翼状螺旋/叉头转录因子2(Foxl2)基因表达的影响,结果表明,50μg/g 剂量的来曲唑对胡子鲇性腺分化无显著影响；但100μg/g和200μg/g剂量的来曲唑可促进胡子鲇精巢分化,抑制卵巢分化,卵巢腔最早出现时间和初级卵母细胞出现时间均分别推迟3d和6d,而初级精母细胞最早出现时间则分别提前2d和5d,且雄性率分别达65.8%和71.3%,显著高于50μg/L组和对照组(P<0.05)。胡子鲇性腺分化前(出膜后12 d), Cyp19a1b和Foxl2基因即开始表达,性腺分化前后Cyp19a1b相对表达量无显著差异(P>0.05),但Foxl2相对表达量则随性分化而逐渐降低,且来曲唑显著抑制性腺分化过程中Cyp19a1b和Foxl2的表达(P<0.05)。结果表明,100μg/g 以上剂量的来曲唑可有效诱导胡子鲇分化为雄性；Cyp19a1b可能不直接参与胡子鲇性腺分化,但Foxl2直接参与此过程。
採用組織學和熒光實時定量PCR方法,檢測飼餵不同劑量芳香化酶抑製劑來麯唑(letrozole,50、100和200μg/g)對鬍子鲇(Clarias fuscus)性腺組織學和性彆比率的影響,以及200μg/g 來麯唑對性分化前後腦型芳香化酶基因(Cyp19a1b)和翼狀螺鏇/扠頭轉錄因子2(Foxl2)基因錶達的影響,結果錶明,50μg/g 劑量的來麯唑對鬍子鲇性腺分化無顯著影響；但100μg/g和200μg/g劑量的來麯唑可促進鬍子鲇精巢分化,抑製卵巢分化,卵巢腔最早齣現時間和初級卵母細胞齣現時間均分彆推遲3d和6d,而初級精母細胞最早齣現時間則分彆提前2d和5d,且雄性率分彆達65.8%和71.3%,顯著高于50μg/L組和對照組(P<0.05)。鬍子鲇性腺分化前(齣膜後12 d), Cyp19a1b和Foxl2基因即開始錶達,性腺分化前後Cyp19a1b相對錶達量無顯著差異(P>0.05),但Foxl2相對錶達量則隨性分化而逐漸降低,且來麯唑顯著抑製性腺分化過程中Cyp19a1b和Foxl2的錶達(P<0.05)。結果錶明,100μg/g 以上劑量的來麯唑可有效誘導鬍子鲇分化為雄性；Cyp19a1b可能不直接參與鬍子鲇性腺分化,但Foxl2直接參與此過程。
채용조직학화형광실시정량PCR방법,검측사위불동제량방향화매억제제래곡서(letrozole,50、100화200μg/g)대호자점(Clarias fuscus)성선조직학화성별비솔적영향,이급200μg/g 래곡서대성분화전후뇌형방향화매기인(Cyp19a1b)화익상라선/차두전록인자2(Foxl2)기인표체적영향,결과표명,50μg/g 제량적래곡서대호자점성선분화무현저영향；단100μg/g화200μg/g제량적래곡서가촉진호자점정소분화,억제란소분화,란소강최조출현시간화초급란모세포출현시간균분별추지3d화6d,이초급정모세포최조출현시간칙분별제전2d화5d,차웅성솔분별체65.8%화71.3%,현저고우50μg/L조화대조조(P<0.05)。호자점성선분화전(출막후12 d), Cyp19a1b화Foxl2기인즉개시표체,성선분화전후Cyp19a1b상대표체량무현저차이(P>0.05),단Foxl2상대표체량칙수성분화이축점강저,차래곡서현저억제성선분화과정중Cyp19a1b화Foxl2적표체(P<0.05)。결과표명,100μg/g 이상제량적래곡서가유효유도호자점분화위웅성；Cyp19a1b가능불직접삼여호자점성선분화,단Foxl2직접삼여차과정。
In this study, Clarias fuscus, a common freshwater fish in China, were selected to determine the effects of an aromatase inhibitor on sex differentiation and related gene expression. Two-day-old juvenile C. fuscus were fed different doses of the aromatase inhibitor, letrozole (50, 100, and 200 μg/g diets) for 30 days. The effects of letrozoleon sex ratio, gonad histology, and Foxl2 and Cyp19a1b expression were examined by morphological ob-servation, histology, and real-time fluorescent quantitative PCR during the period of sex differentiation (12?30 d after hatching). The results showed that letrozole doses of 100 μg/g and 200 μg/g produced more males (65.9%and 71.3%, respectively) than did a dose of 50 μg/g (64.7%) (P<0.05). However, no significant difference in sex ratio was observed between the 50 μg/L 17α-MT treated group and the control group (51.1%). In addition, letrozole accelerated the occurrence of primary spermatocytes for 2 and 5 days, but deferred that of ovarian cavity and primary oocytes for 3 and 6 days, respectively. Both Cyp19a1b and Foxl2 were expressed prior to sex differ-entiation in C. fuscus. No difference was observed in the level of Cyp19a1b expression prior to and post sex dif-ferentiation (12?30 d after hatching). However, Foxl2 expression decreased with gonadal differentiation. In addi-tion, letrozole at dose of 200 μg/g inhibited the expression of both Cyp19a1b and Foxl2 (P<0.05). Our results suggest that letrozole doses above 100μg/g could induce C. fuscus masculinization, and that Cyp19a1b is not di-rectly involved in the process of gonadal differentiation but Foxl2 is.
