CAJ | 학술논문

为建立紫茉莉(Mirabilis jalapa L.)悬浮细胞培养体系，以紫茉莉无菌苗叶片诱导的愈伤组织为材料，筛选紫茉莉悬浮细胞的适宜培养体系。结果表明，紫茉莉愈伤组织在MS+2,4-D 1 mg L-1+KT 0.5 mg L-1的液体培养基中悬浮继代培养3~4次，能得到稳定的悬浮细胞系。培养基的pH值为5.5~5.9，蔗糖浓度为30 g L-1更适合悬浮细胞的生长。紫茉莉悬浮细胞的生长曲线大致呈S型。最佳继代培养时间是10 d，培养液的体积为40 mL时，接种量为7.5 mL，可以较好地保持悬浮细胞系。1 L培养液中可提取分泌蛋白(0.42±0.15) g。这些有助于对悬浮细胞提取分泌蛋白的研究。
위건립자말리(Mirabilis jalapa L.)현부세포배양체계，이자말리무균묘협편유도적유상조직위재료，사선자말리현부세포적괄의배양체계。결과표명，자말리유상조직재MS+2,4-D 1 mg L-1+KT 0.5 mg L-1적액체배양기중현부계대배양3~4차，능득도은정적현부세포계。배양기적pH치위5.5~5.9，자당농도위30 g L-1경괄합현부세포적생장。자말리현부세포적생장곡선대치정S형。최가계대배양시간시10 d，배양액적체적위40 mL시，접충량위7.5 mL，가이교호지보지현부세포계。1 L배양액중가제취분비단백(0.42±0.15) g。저사유조우대현부세포제취분비단백적연구。
In order to establish suspension cell system of Mirabilis jalapa L., the calli induced from leaves of aseptic seedlings of M. jalapa were used as material, the optimum suspension culture system was studied. The results showed that the stable suspension cell system could be obtained after calli were subcultured 3-4 times on MS medium with 2,4-D 1 mg L-1 and KT 0.5 mg L-1. The optimum pH was 5.5-5.9 with 30 g L-1 sucrose. The growth curve of suspension cells of M. jalapa was S-type, and subculture time was about 10 days. The optimum inoculum was 7.5 mL in 40 mL medium. Secretory protein was (0.42 ± 0.15) g extracted from 1 L medium. These were useful for studying secretory protein extracted from suspension system.