CAJ | 학술논문

为了解扇脉杓兰(Cypripedium japonicum Thunb.)和无距虾脊兰(Calanthe tsoongiana T. Tang et F. T. Wang)的核型，采用根尖压片法对扇脉杓兰和无距虾脊兰的染色体数目和核型进行了研究。结果表明，扇脉杓兰体细胞的染色体数为22，核型公式为2n=2x=22=16m+2sm+2st+2t，染色体相对长度组成为2n=22=2L+6M2+12M1+2S，核不对称系数为60.01%，核型分类为2B型；而无距虾脊兰体细胞的染色体数为40，核型公式为2n=2x=40=28m+10sm+2st，染色体相对长度组成为2n=40=8L+10M2+16M1+6S，核不对称系数为59.84%，核型分类为2B型；两者核型都较为对称。其中，无距虾脊兰的核型为首次报道。这为扇脉杓兰和无距虾脊兰的进化地位和种质保护提供了细胞学证据。
위료해선맥표란(Cypripedium japonicum Thunb.)화무거하척란(Calanthe tsoongiana T. Tang et F. T. Wang)적핵형，채용근첨압편법대선맥표란화무거하척란적염색체수목화핵형진행료연구。결과표명，선맥표란체세포적염색체수위22，핵형공식위2n=2x=22=16m+2sm+2st+2t，염색체상대장도조성위2n=22=2L+6M2+12M1+2S，핵불대칭계수위60.01%，핵형분류위2B형；이무거하척란체세포적염색체수위40，핵형공식위2n=2x=40=28m+10sm+2st，염색체상대장도조성위2n=40=8L+10M2+16M1+6S，핵불대칭계수위59.84%，핵형분류위2B형；량자핵형도교위대칭。기중，무거하척란적핵형위수차보도。저위선맥표란화무거하척란적진화지위화충질보호제공료세포학증거。
The aim was to understand the karyotype of Cypripedium japonicum Thunb. and Calanthe tsoongiana T. Tang et F. T. Wang. Their chromosome number and karyotype were studied by using ifngertip squashing method. The results showed that the chromosome number of Cypripedium japonium was 2n = 22, karyotype formula was 2n=2x=22=16m+2sm+2st+2t, constitution of relative length was 2n=22=2L+6M2+12M1+2S, asymmetry index was 60.01%, karyotype type was classiifed as 2B;while the chromosome number of Calanthe tsoongiana was 2n=40, karyotype formula was 2n=2x=40=28m+10sm+2st, constitution of relative length was 2n = 40 = 8L+ 10M2+ 16M1+ 6S, asymmetry index was 59.84%, karyotype type also belonged to 2B. Both of them have relatively symmetric karyotype. The karyotype of Calanthe tsoongiana was reported for the first time. These provided some cytological evidences for evolutionary status and germplasm conservation of Cypripedium japonicum and Calanthe tsoongiana.