CAJ | 학술논문

为探讨福建省野牡丹属(Melastoma L.)植物的亲缘关系，运用ISSR分子标记技术对源自福建省的野牡丹属野生种质及部分实生后代共34份材料进行分析。结果表明，11条多态性引物对34份种质DNA进行扩增，共获得112条完整、清晰的谱带，其中多态性条带104条，多态性比率为92.9%，表明野牡丹属种质资源具有较高的遗传多样性。34份种质材料的相似系数为0.55~0.93，平均相似系数为0.71，表明这些材料间的亲缘关系较近。聚类分析表明34份材料可划分为3个类群5个亚类，主坐标散点分析可分为4个类群，这与亲缘关系分析的结果基本一致。这从分子水平上揭示了福建省野牡丹属野生种质及部分具备优良园艺性状实生后代的亲缘关系，为该属植物的良种选育工作提供了理论依据。
위탐토복건성야모단속(Melastoma L.)식물적친연관계，운용ISSR분자표기기술대원자복건성적야모단속야생충질급부분실생후대공34빈재료진행분석。결과표명，11조다태성인물대34빈충질DNA진행확증，공획득112조완정、청석적보대，기중다태성조대104조，다태성비솔위92.9%，표명야모단속충질자원구유교고적유전다양성。34빈충질재료적상사계수위0.55~0.93，평균상사계수위0.71，표명저사재료간적친연관계교근。취류분석표명34빈재료가화분위3개류군5개아류，주좌표산점분석가분위4개류군，저여친연관계분석적결과기본일치。저종분자수평상게시료복건성야모단속야생충질급부분구비우량완예성상실생후대적친연관계，위해속식물적량충선육공작제공료이론의거。
In order to understand the relationship of Melastoma, the DNA ifngerprints of 34 Melastoma germplasm collected from different regions of Fujian Province were studied by using ISSR markers. Eleven primers selected from 116 ISSR primers could obtain high polymorphism and reproducibility bands. The results showed that total of 112 DNA bands were ampliifed, and 104 polymorphic bands, counting for 92.9%. Genetic similarity analysis showed that for 34 Melastoma germplasm the Nei’s coefifcient of 34 Melastoma germplasm ranged from 0.55 to 0.93, with the average of 0.71. It was suggested that there were close genetic relationships and high genetic diversity among Melastoma germplasm resources. These 34 germplasm resources could divided into 3 groups and 5 subgroups by UPGMA analysis, and 4 groups by principal coordinate scatter analysis. These results had high consistency with that of genetic relationship analysis. Therefore, it was suggested that ISSR markers could be used to reveal genetic diversity of Melastoma at molecular level, and provided a theoretical basis for breeding of Melastoma in Fujian.