CAJ | 학술논문

目的探讨T-bet、GATA-3和Foxp3在复发性阿弗他溃疡（RAU）发病机制中的作用及意义。方法选择RAU患者33例及健康对照30例，采用逆转录聚合酶链反应技术检测患者溃疡发作期外周血单个核细胞（PBMC）中T-bet、GATA-3和Foxp3 mRNA的表达水平，并分析其与RAU免疫功能之间的关系。结果 RAU患者的CD4、CD4/8、T+B+NK细胞含量低于正常值（P<0.01），免疫球蛋白（Ig）A低于正常值（P<0.05），而IgM、血清总补体活性高于正常值（P<0.05）。RAU患者PBMC中T-bet mRNA、T-bet/GATA-3比值、Foxp3 mRNA低于对照组（P<0.01），GATA-3 mRNA高于对照组（P<0.05）；Foxp3表达与T-bet/GATA-3比值之间存在正相关关系（r=0.404，P=0.02）。结论 RAU患者大多存在细胞免疫降低和体液免疫紊乱的问题；其溃疡发作期PBMC中GATA-3表达上升，T-bet表达下降，促进了辅助性T细胞（Th）2优势应答，可能是导致RAU患者免疫功能紊乱的原因之一；而调节性T细胞的数量减少及功能低下，这可能与RAU患者Th1/Th2细胞失衡有关。
목적탐토T-bet、GATA-3화Foxp3재복발성아불타궤양（RAU）발병궤제중적작용급의의。방법선택RAU환자33례급건강대조30례，채용역전록취합매련반응기술검측환자궤양발작기외주혈단개핵세포（PBMC）중T-bet、GATA-3화Foxp3 mRNA적표체수평，병분석기여RAU면역공능지간적관계。결과 RAU환자적CD4、CD4/8、T+B+NK세포함량저우정상치（P<0.01），면역구단백（Ig）A저우정상치（P<0.05），이IgM、혈청총보체활성고우정상치（P<0.05）。RAU환자PBMC중T-bet mRNA、T-bet/GATA-3비치、Foxp3 mRNA저우대조조（P<0.01），GATA-3 mRNA고우대조조（P<0.05）；Foxp3표체여T-bet/GATA-3비치지간존재정상관관계（r=0.404，P=0.02）。결론 RAU환자대다존재세포면역강저화체액면역문란적문제；기궤양발작기PBMC중GATA-3표체상승，T-bet표체하강，촉진료보조성T세포（Th）2우세응답，가능시도치RAU환자면역공능문란적원인지일；이조절성T세포적수량감소급공능저하，저가능여RAU환자Th1/Th2세포실형유관。
Objective??To?elucidate?the?underlying?role?of?T-bet,?GATA-3,?and?Foxp3?in?the?immune?etiology?of?human?with?recurrent?aphthous?ulcer(RAU).?Methods???Peripheral?blood?mononuclear?cells(PBMCs)?were?isolated?by?density?gradient?centrifugation?from?33?cases?of?RAU?and?30?cases?of?healthy?controls.?The?expression?levels?of?T-bet,?GATA-3,?and?Foxp3?mRNA?in?PBMCs?were?investigated?by?reverse?transcription-polymerase?chain?reaction.?The?cellular?and?humoral?immune?status?of?patients?with?RAU?was?investigated?by?flow?cytometry?and?enzyme-linked?immunosorbent?assay.?Results??CD4,?CD4/CD8,?T+B+NK?cells?were?significantly?lower?in?the?RAU?group?than?in?the?control?group(P<0.01).?Immunoglobulin(Ig)?A?was?lower?in?the?RAU?group?than?in?the?control?group(P<0.05).?IgM?and?50%?hemolytic?complement?were?significantly?higher?in?the?RAU?group?than?in?the?control?group(P<0.05).?The?expression?level?of?T-bet?mRNA?and?Foxp3?mRNA,?the?ratio?of?T-bet/GATA-3?in?the?RAU?group?were?lower?than?that?in?the?control?group(P<0.01).?Meanwhile,?the?expression?level?of?GATA-3?mRNA?in?the?RAU?group?was?higher?than?that?in?the?control?group(P<0.05).?A?positive?correlation?was?detected?between?Foxp3?mRNA?level?and?T-bet/GATA-3?ratio(r=0.404,?P=0.02).?Conclusion?Most?RAU?patients?have?immune?cells?reduced?and?humoral?immune?disorder.?The?increased?expression?of?GATA-3?and?decreased?expression?of?T-bet?might?be?one?of?the?mechanisms?of?T?helper?cell(Th)1/Th2?imbalance?in?RAU,?accounting?for?the?abnormal?immune?function?in?patients?with?RAU.?The?decrease?in?regulatory?T?cells?might?be?a?contributing?factor?toward?Th1/Th2?imbalance?in?RAU.