中国骨伤
中國骨傷
중국골상
CHINA JOURNAL OF ORTHOPAEDICS AND TRAUMATOLOGY
2013年
8期
689-693
,共5页
苦碟子%外科皮瓣%血管内皮生长因子类%新生血管,生理性
苦碟子%外科皮瓣%血管內皮生長因子類%新生血管,生理性
고설자%외과피판%혈관내피생장인자류%신생혈관,생이성
Sowthistle leaf ixeris seedling%Surgical flaps%Vascular endothelial growth factors (VEGF)%Neovascu-larization,physiologic
目的：观察苦碟子注射液研究苦碟子注射液对大鼠随意型皮瓣存活的影响对大鼠随意皮瓣成活的影响。方法：将2～3月龄重SD大鼠24只，采用随机抽签法分为2组（实验组和对照组，每组12只）。采用改良的大鼠随意型皮瓣制作方法造模，实验组每天腹腔注射苦碟子注射液5 ml/kg ，对照组每天腹腔注射生理盐水5 ml/kg。术后第7天颈椎脱臼处死大鼠分别进行皮瓣存活面积比的检测，同时取皮瓣近中远端组织经组织染色切片后光镜下观察，免疫组化法检测血管内皮生长因子（VEGF）的表达。结果：术后7 d，实验组皮瓣存活面积比为（70．432±3．867）％，显著高于对照组的（50．498±2．346）％（P＜0．01）；实验组皮瓣组织水肿、炎症细胞浸润情况比对照组明显减轻；切片观察出现较多新生血管，Ⅱ区新生血管密度（（30．11±5．53）/mm2，与对照组（20．13±4．11））/mm2比较，差异有统计学意义（P＜0．01），Ⅲ区坏死严重。实验组VEGF表达量为（4867．31±452．36），与对照组的VEGF表达量（2387．45±768．46）比较，差异有统计学意义（P＜0．01）。结论：苦碟子注射液可以提高VEGF的表达量、促进毛细血管新生，减轻皮瓣炎性浸润，还可以明显提高大鼠随意型皮瓣的成活率，为临床皮瓣移植研究提供了新的思路。
目的：觀察苦碟子註射液研究苦碟子註射液對大鼠隨意型皮瓣存活的影響對大鼠隨意皮瓣成活的影響。方法：將2～3月齡重SD大鼠24隻，採用隨機抽籤法分為2組（實驗組和對照組，每組12隻）。採用改良的大鼠隨意型皮瓣製作方法造模，實驗組每天腹腔註射苦碟子註射液5 ml/kg ，對照組每天腹腔註射生理鹽水5 ml/kg。術後第7天頸椎脫臼處死大鼠分彆進行皮瓣存活麵積比的檢測，同時取皮瓣近中遠耑組織經組織染色切片後光鏡下觀察，免疫組化法檢測血管內皮生長因子（VEGF）的錶達。結果：術後7 d，實驗組皮瓣存活麵積比為（70．432±3．867）％，顯著高于對照組的（50．498±2．346）％（P＜0．01）；實驗組皮瓣組織水腫、炎癥細胞浸潤情況比對照組明顯減輕；切片觀察齣現較多新生血管，Ⅱ區新生血管密度（（30．11±5．53）/mm2，與對照組（20．13±4．11））/mm2比較，差異有統計學意義（P＜0．01），Ⅲ區壞死嚴重。實驗組VEGF錶達量為（4867．31±452．36），與對照組的VEGF錶達量（2387．45±768．46）比較，差異有統計學意義（P＜0．01）。結論：苦碟子註射液可以提高VEGF的錶達量、促進毛細血管新生，減輕皮瓣炎性浸潤，還可以明顯提高大鼠隨意型皮瓣的成活率，為臨床皮瓣移植研究提供瞭新的思路。
목적：관찰고설자주사액연구고설자주사액대대서수의형피판존활적영향대대서수의피판성활적영향。방법：장2～3월령중SD대서24지，채용수궤추첨법분위2조（실험조화대조조，매조12지）。채용개량적대서수의형피판제작방법조모，실험조매천복강주사고설자주사액5 ml/kg ，대조조매천복강주사생리염수5 ml/kg。술후제7천경추탈구처사대서분별진행피판존활면적비적검측，동시취피판근중원단조직경조직염색절편후광경하관찰，면역조화법검측혈관내피생장인자（VEGF）적표체。결과：술후7 d，실험조피판존활면적비위（70．432±3．867）％，현저고우대조조적（50．498±2．346）％（P＜0．01）；실험조피판조직수종、염증세포침윤정황비대조조명현감경；절편관찰출현교다신생혈관，Ⅱ구신생혈관밀도（（30．11±5．53）/mm2，여대조조（20．13±4．11））/mm2비교，차이유통계학의의（P＜0．01），Ⅲ구배사엄중。실험조VEGF표체량위（4867．31±452．36），여대조조적VEGF표체량（2387．45±768．46）비교，차이유통계학의의（P＜0．01）。결론：고설자주사액가이제고VEGF적표체량、촉진모세혈관신생，감경피판염성침윤，환가이명현제고대서수의형피판적성활솔，위림상피판이식연구제공료신적사로。
Objective:To observe the effects of Ixeri sonhifolia injection on random skin flap survival in rats. Methods:Dorsal full thickness skin flap model were harvested from 24 Sprague-Dawley rats in 2 to 3 months old. Twelve rats in experi-mental group were injected intraperitoneally with 5 ml/kg Ixeri sonhifolia injection immediately after the operation ,the other rats in control group with an equal volume of saline. The rats were killed by cervical dislocation after 7 days'Ixeri sonhifolia in-jection. The area ratio of the survival tissue was measured at the 7th day,the tissue samples from proximal,middle,and distal portions were stained by HE and sectioned for histological and image analysis. VEGF was detected by immunohistochemistry. Results:Seven days later,there was statistical significance between the percentage of the survival area of the flap between the experimental group (70.432 ±3.867)%and the control group (50.498±2.346)% (P<0.05). In the middle portion,edema and infiltration of tissue in the experimental group were reduced than those of the control group ,and new blood vessels in-creased in the experimental group (P<0.05). A statistical significance of the expression of VEGF was detected between experi-mental group (4867.31±452.36) and control group (2387.45±768.46)(P<0.05). Conclusion:Ixeri sonhifolia injection can promote the survival of random skin flap by increasing the quantity of capillary ,reducing inflammatory infiltration of Europhiles and increasing the expression of VEGF,which promote a new approaching for the transpanting of the random flap research.
