猪肾细胞系IBRS-2感染猪细小病毒的检测
저신세포계IBRS-2감염저세소병독적검측
Detection of porcine parvovirus infection in porcine kidney cell line IBRS-2
  • 万方数据
    中国动物检疫 중국동물검역
    CHINA ANMAL QUARANTINE
    2013年 8期 67-69,74 ,共4页

    李斌%梁家幸%苏乾莲%赵武%秦毅斌%何颖%卢冰霞%段群棚%李莹莹%姜佳佳%梁保忠

    리빈%량가행%소건련%조무%진의빈%하영%로빙하%단군붕%리형형%강가가%량보충

    聚合酶链式反应%猪细小病毒%猪肾细胞系IBRS-2%检测 聚閤酶鏈式反應%豬細小病毒%豬腎細胞繫IBRS-2%檢測
    취합매련식반응%저세소병독%저신세포계IBRS-2%검측
    polymerase chain reaction (PCR)%porcine parvovirus (PPV)%porcine kidney cell line IBRS-2%detection
    本研究应用聚合酶链式反应技术对未接种猪细小病毒，但出现类似猪细小病毒感染病变的日常培养猪肾细胞系IBRS-2进行检测，结果发现IBRS-2细胞感染了猪细小病毒。检测结果表明猪肾细胞系IBRS-2感染猪细小病毒较为常见，提示在IBRS-2细胞培养过程中要注意预防猪细小病毒的感染。
    본연구응용취합매련식반응기술대미접충저세소병독，단출현유사저세소병독감염병변적일상배양저신세포계IBRS-2진행검측，결과발현IBRS-2세포감염료저세소병독。검측결과표명저신세포계IBRS-2감염저세소병독교위상견，제시재IBRS-2세포배양과정중요주의예방저세소병독적감염。
    In the study, porcine parvovirus (PPV) was detected in daily cultured porcine kidney cell line IBRS-2, which had not been inoculated with PPV, but showed PPV cytopathic, by polymerase chain reaction (PCR). The detection result showed that these IBRS-2 cells were infected by PPV. Detection result suggested that porcine kidney cell line IBRS-2 infected by PPV was common. We should pay attention to prevention of PPV contamination in cell culture.
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