中国动物检疫
中國動物檢疫
중국동물검역
CHINA ANMAL QUARANTINE
2013年
8期
63-64
,共2页
蔡其刚%徐天刚%马利青
蔡其剛%徐天剛%馬利青
채기강%서천강%마리청
弓形虫病%重组蛋白GST-SAG1%ELISA%喜马拉雅旱獭
弓形蟲病%重組蛋白GST-SAG1%ELISA%喜馬拉雅旱獺
궁형충병%중조단백GST-SAG1%ELISA%희마랍아한달
Toxoplasmosis%Recombinant Protein GST-SAG1%ELISA%Himalayan Marmota
用弓形虫的重组蛋白GST-SAG1作为ELISA诊断抗原,进行了ELISA诊断方法的建立,并对青海省喜马拉雅旱獭进行了弓形虫病的血清学调查。经对所收集的68份喜马拉雅旱獭血清抗体进行检测,共检出阳性血清15份,阳性率约为22.06%,表明青海省喜马拉雅旱獭中存在弓形虫病的感染。
用弓形蟲的重組蛋白GST-SAG1作為ELISA診斷抗原,進行瞭ELISA診斷方法的建立,併對青海省喜馬拉雅旱獺進行瞭弓形蟲病的血清學調查。經對所收集的68份喜馬拉雅旱獺血清抗體進行檢測,共檢齣暘性血清15份,暘性率約為22.06%,錶明青海省喜馬拉雅旱獺中存在弓形蟲病的感染。
용궁형충적중조단백GST-SAG1작위ELISA진단항원,진행료ELISA진단방법적건립,병대청해성희마랍아한달진행료궁형충병적혈청학조사。경대소수집적68빈희마랍아한달혈청항체진행검측,공검출양성혈청15빈,양성솔약위22.06%,표명청해성희마랍아한달중존재궁형충병적감염。
The purified recombinant protein GST-SAG1 of Toxoplasma gondii was used to establish an ELISA for diagnosis of Toxoplasma antibodies,and 68 serum samples collected from Himalayan Marmota in Qinghai province were used to detect the antibodies against Toxoplasma gondii by the established ELISA method. The results showed that 15 serum samples were positive and the positive rate was 22.06%, sug-gesting that Toxoplasma gondii has infected Himalayan Marmots in Qinghai province.