CAJ | 학술논문

目的探讨乳腺癌患者手术前后外周血淋巴细胞表型CD8、CD28变化及临床意义．方法应用CD8、CD28单克隆抗体，采用流式细胞术，对40例乳腺癌和25例对照组手术前后 CD8， CD8+CD28+和CD8+CD28-进行测定．结果乳腺癌 CD8的表达（32.8±4.5）%高于对照组（30.4±3.3）%， P＜0.05，而CD8+CD28+细胞表达（10.4±1.7）%则低于对照组（14.3±2.6）%，CD8+CD28-细胞表达（25.1±2.8）%高于对照组（19.6±2.3）%，P＜0.01.切除肿瘤后，外周血CD8、CD8+CD28-细胞、CD8+CD28+测定值（31.9±4.3）%（15.4±2.7）%（20.4±2.8）%与正常对照组相比无明显变化， P＞0.05．手术前后 CD8、CD8+CD28-细胞、CD8+CD28+细胞测定值均有差异P＜0.05．结论乳腺癌手术切除后，细胞毒性T淋巴细胞免疫防御和免疫监视增强，免疫抑制减弱，减少肿瘤免疫逃逸，对乳腺癌免疫治疗和预后判断具有一定的指导意义．
목적탐토유선암환자수술전후외주혈림파세포표형CD8、CD28변화급림상의의．방법응용CD8、CD28단극륭항체，채용류식세포술，대40례유선암화25례대조조수술전후 CD8， CD8+CD28+화CD8+CD28-진행측정．결과유선암 CD8적표체（32.8±4.5）%고우대조조（30.4±3.3）%， P＜0.05，이CD8+CD28+세포표체（10.4±1.7）%칙저우대조조（14.3±2.6）%，CD8+CD28-세포표체（25.1±2.8）%고우대조조（19.6±2.3）%，P＜0.01.절제종류후，외주혈CD8、CD8+CD28-세포、CD8+CD28+측정치（31.9±4.3）%（15.4±2.7）%（20.4±2.8）%여정상대조조상비무명현변화， P＞0.05．수술전후 CD8、CD8+CD28-세포、CD8+CD28+세포측정치균유차이P＜0.05．결론유선암수술절제후，세포독성T림파세포면역방어화면역감시증강，면역억제감약，감소종류면역도일，대유선암면역치료화예후판단구유일정적지도의의．
Objective To investigate the expression of CD8 and CD28 lymphocytes in peripheral blood of patients with breast cancer before and after operation and explore its clinical significance.Method Flow cytometry was applied to test the expression of CD8,CD8+CD28+and D8+CD28-in the peripheral blood of both 40 patients with breast cancer and 25 healthy controls. Results The expression levels of CD8 in patients with breast cancer were significantly higher than those in healthy controls [(30.4±3.3)%VS. 30.4±3.3)%,P<0.05] . The expression levels of CD8+CD28+in patients with breast cancer were significantly lower than those in healthy controls [(10.4 ± 1.7)%VS. (14.3±2.6)%,P<0.05] . The expression levels of CD8+CD28-in patients with breast cancer were significantly higher than those in healthy controls [(25.1 ±2.8)% VS. (19.6 ±2.3)%, P<0.05] . After operation, the expression levels of CD8, CD8+CD28+and CD8+CD28-[(31.9 ±4.3)%, (15.4 ±2.7)%and (20.4±2.8)%, respectively] in peripheral blood of patients with breast cancer had no significant change compared with the healthy controls. The expression levels of CD8, CD8+CD28+and CD8+CD28-in peripheral blood of patients with breast cancer had significant difference between before and after operation. Conclusions After surgical operation, the immune defence and immune surveillance of cytotoxic T lymphocytes can be enhanced, immune suppression and tumor escape can be reduced. The assays of cell phenotypes CD8 and CD28 might be useful for evaluating the immunal state and prognosis of patient s with breast cancer.