CAJ | 학술논문

建立高效液相色谱-串联质谱法测定纺织品中芦荟苷、芦荟大黄素和大黄酚含量的方法。样品经甲醇60℃超声波浴提取30 min后，在Agilent Eclipse XDB-C18（2．1 mm ×150 mm，3．5 m）色谱柱上分离，以10 mmol/L乙酸铵溶液（pH＝5．75）和乙腈为流动相进行梯度洗脱。三重四级杆质谱采用电喷雾离子源负离子模式在多反应监测（ MRM）模式下检测被分析物，外标法定量。纺织样品在3个加标水平时，平均回收率为85．5％～99．9％，相对标准偏差（ RSDs）为1．76％～5．92％。该方法具有快速、灵敏、准确、可重复的特点，可应用于纺织品中芦荟成分的痕量分析。
건립고효액상색보-천련질보법측정방직품중호회감、호회대황소화대황분함량적방법。양품경갑순60℃초성파욕제취30 min후，재Agilent Eclipse XDB-C18（2．1 mm ×150 mm，3．5 m）색보주상분리，이10 mmol/L을산안용액（pH＝5．75）화을정위류동상진행제도세탈。삼중사급간질보채용전분무리자원부리자모식재다반응감측（ MRM）모식하검측피분석물，외표법정량。방직양품재3개가표수평시，평균회수솔위85．5％～99．9％，상대표준편차（ RSDs）위1．76％～5．92％。해방법구유쾌속、령민、준학、가중복적특점，가응용우방직품중호회성분적흔량분석。
An ultra effective liquid chromatography-tandem mass spectrometry ( LC-MS/MS ) method has been developed for determinating the contents of aloin , aloe-emodin and chrysophanol in textiles .The samples were prepared by ultrasonically extracting using methanol at 60 ℃for 30 min.The LC separation was performed on an Agilent XDB-C18 column (2.1 mm ×150 mm, 3.5 m) using 10 mmol/L ammonium acetate as solution (pH=5.75) and acetonitrile as the mobile phase in a gradient elution mode .The analyses were tested by a triple-quadrupole mass spectrometer in the multiple reaction monitoring ( MRM) mode using electrospray source with negative mode , and quantified by external standard method .The average recoveries of analyses in textile samples at the three spiked levels were in the range of 85.5%~99.9%with the relative standard deviations being 1.76%~5.92%.The method was proved to be rapid , sensitive , accurate and reproducible and can be applied to the trace analysis of aloe constituents in textiles.