CAJ | 학술논문

目的：该研究从整体水平比较microRNA（miRNA）对管家基因和非管家基因的调控差异并研究3′UTR的进化保守性与miRNA对两类基因调控差异之间的相关性。方法通过对3个基于序列的miRNA靶基因预测软件整合分析，并结合基于miRNA-靶基因的表达谱数据相关性分析，以获得miRNA对两类基因的调控情况，同时利用phastCons保守性分值对两类基因的3′UTR区域进行多物种进化分析。结果研究发现miRNA在管家基因的3′UTR上有显著地高密度绑定，管家基因的3′UTR区域具有相对较高的序列保守性。结论该研究结果突出了miRNA对管家基因表达调控的重要作用，对于重新理解miRNA对真核生物基因表达调控作用具有一定的参考价值。
목적：해연구종정체수평비교microRNA（miRNA）대관가기인화비관가기인적조공차이병연구3′UTR적진화보수성여miRNA대량류기인조공차이지간적상관성。방법통과대3개기우서렬적miRNA파기인예측연건정합분석，병결합기우miRNA-파기인적표체보수거상관성분석，이획득miRNA대량류기인적조공정황，동시이용phastCons보수성분치대량류기인적3′UTR구역진행다물충진화분석。결과연구발현miRNA재관가기인적3′UTR상유현저지고밀도방정，관가기인적3′UTR구역구유상대교고적서렬보수성。결론해연구결과돌출료miRNA대관가기인표체조공적중요작용，대우중신리해miRNA대진핵생물기인표체조공작용구유일정적삼고개치。
Objective To study the microRNA ( miRNA) regulation on the expression of housekeeping and non-house-keeping human genes from the systemic level , and to reveal the influence of conservation at 3′UTRs region for the density difference of miRNA regulatory between the two classes of genes .Methods We obtained miRNA regulation informations for the two classes of genes through the integration analysis of three sequence-based target gene prediction softwares and the conjoint analysis based on gene expression profile data .We also used phastCons-score based evolution analysis to study the conservation of 3′UTRs regions between the two classes of genes .Results Our results showed that housekeeping genes have a significantly higher density of miRNA binding sites and their 3′UTR are relatively conservative .Conclusion These findings highlight the importance of miRNA regulation in housekeeping genes , suggesting that investigating the roles of miR-NAs on the robustness of housekeeping genes expression is highly necessary .