CAJ | 학술논문

目的：分析肝硬化患者远端肠道微生态菌群代谢功能的变化。方法选取16例肝硬化患者及20例正常人，提取其肠道微生物宏基因组DNA进行高通量Solexa测序，对测序基因进行代谢功能的注释，比较肝硬化患者与正常人之间的差异，找出疾病相关的肠道微生物代谢功能的变化。结果肝硬化患者肠道菌群功能多样性降低，对药物、必需氨基酸、丙酸盐等的代谢能力以及炎性反应显著增强，而对丁酸盐、胆汁酸的代谢能力以及细胞周期相关的功能显著降低。结论在肝硬化的影响下，肠道微生物的生长环境被破坏，肠道菌群为了适应环境，在功能和代谢方面表现出一定程度的代偿。
목적：분석간경화환자원단장도미생태균군대사공능적변화。방법선취16례간경화환자급20례정상인，제취기장도미생물굉기인조DNA진행고통량Solexa측서，대측서기인진행대사공능적주석，비교간경화환자여정상인지간적차이，조출질병상관적장도미생물대사공능적변화。결과간경화환자장도균군공능다양성강저，대약물、필수안기산、병산염등적대사능력이급염성반응현저증강，이대정산염、담즙산적대사능력이급세포주기상관적공능현저강저。결론재간경화적영향하，장도미생물적생장배경피파배，장도균군위료괄응배경，재공능화대사방면표현출일정정도적대상。
Objective To investigate the metabolism and function of the intestinal microbiota from liver cirrhosis patients.Methods Sixteen cases of liver cirrhosis and twenty normal individuals were selected , whose intestinal microbiota metagenomic DNA was extracted , followed by high-throughput Solexa sequencing and the bioinformatics analysis of metabo-lism and function annotation to compare the differences between the patients and normal subjects and find out about the cir -rhosis-related functions .Results The functional diversity was significantly reduced in the intestinal microbiota of cirrhotic patients.At the module or pathway level , the intestinal microbiota of patients showed an enrichment in metabolisms of drugs, essential amino acid , propanoate metabolism and inflammatory reaction , whereas an opposite tendency was observed in the metabolic ability of butyrate , bile acid and cell cycle .Conclusion Under the influence of liver cirrhosis , the growth environment in the intestine is destroyed , causing, the intestinal microbiota the exhibit some compensation to adapt to the changed intestinal micro-environment .