CAJ | 학술논문

背景与目的骨转移占晚期肺癌的50%-70%。本研究以体外侵袭、迁移能力不同的肺腺癌细胞系A549、H1299、SPC-A-1、XL-2为基础建立肺腺癌骨转移裸小鼠模型，MicroCT观察骨转移情况。方法将50只6 w-8 w龄裸小鼠随机平均分为5组，4个实验组左心室分别注射相应四种细胞悬液（0.2 mL/只）；对照组左心室注射等量生理盐水。注射后第二周起定期对各组小鼠进行MicroCT扫描，当小鼠明显消瘦时此组观察结束，结束前行骨组织病理学检查；对各实验组出现的骨转移部位按中轴骨和四肢骨归类，比较这两种部位之间的转移率；根据各组出现骨转移所用平均时间、骨转移率，对各细胞系骨转移能力进行统计分析。结果经MicroCT、病理学检查确定，各实验组出现不同骨转移率，对照组小鼠无骨转移现象；各实验组中轴骨转移率均明显高于四肢骨，这与临床上肺癌骨转移规律一致，模型建立成功。各实验组间发生骨转移的小鼠数目及出现转移所用平均时间无明显差异。结论 MicroCT能清晰地检测到骨质破坏，利于骨转移情况的判断；我们成功建立了肺腺癌骨转移模型，为以后探索出新的肺腺癌乃至肺癌骨转移临床预防和治疗方案提供基础；4种肺腺癌细胞系体外侵袭、迁移能力强弱不等，但体内骨转移能力没有明显差异，其原因还有待进一步的探索。
배경여목적골전이점만기폐암적50%-70%。본연구이체외침습、천이능력불동적폐선암세포계A549、H1299、SPC-A-1、XL-2위기출건립폐선암골전이라소서모형，MicroCT관찰골전이정황。방법장50지6 w-8 w령라소서수궤평균분위5조，4개실험조좌심실분별주사상응사충세포현액（0.2 mL/지）；대조조좌심실주사등량생리염수。주사후제이주기정기대각조소서진행MicroCT소묘，당소서명현소수시차조관찰결속，결속전행골조직병이학검사；대각실험조출현적골전이부위안중축골화사지골귀류，비교저량충부위지간적전이솔；근거각조출현골전이소용평균시간、골전이솔，대각세포계골전이능력진행통계분석。결과경MicroCT、병이학검사학정，각실험조출현불동골전이솔，대조조소서무골전이현상；각실험조중축골전이솔균명현고우사지골，저여림상상폐암골전이규률일치，모형건립성공。각실험조간발생골전이적소서수목급출현전이소용평균시간무명현차이。결론 MicroCT능청석지검측도골질파배，리우골전이정황적판단；아문성공건립료폐선암골전이모형，위이후탐색출신적폐선암내지폐암골전이림상예방화치료방안제공기출；4충폐선암세포계체외침습、천이능력강약불등，단체내골전이능력몰유명현차이，기원인환유대진일보적탐색。
Background and objective 50%-70%of patients with advanced lung cancer will develop bone metas-tases. hTe aim of this study is to establish the nude mice bone metastasis model of lung adenocarcinoma using A549, H1299, SPC-A-1 and XL-2, all of which own different invasion and migration abilities in vitro and supervise the bone metastases by MicroCT. Methods iftfy BALB/C-nu/nu nude mice were grouped into ifve groups on average randomly. Cells of the four cell lines were injected into the letf cardiac ventricle of mice in the four experimental groups (0.2 mL/mouse) respectively;meanwhile, mice in the control group were injected with normal saline (0.2 mL/mouse) in the same manner. Periodical radio-logical examination was carried out to supervise the variation of the mice since the second week atfer injection. When mice in each group became thin obviously, end the experiment of this group. Before the end, pathological sections of bone tissues were made. We classiifed the bone metastatic sites into axial skeleton and limb bone, in order to compare the metastatic rates of these two different parts. hTe bone metastatic abilities of the four cell lines was statistically analyzed by comparing the average time cost in the appearance of bone metastases and the percentage of bone metastases among the experimental groups. Results Different metastatic sites which had been identiifed both by MicroCT and pathological sections appeared in each group of the four experimental groups. By contrast, no metastasis was observed in the control group. hTe percentage of cancer metastasiz-ing to axial skeleton was remarkably higher than the percentage of tumor metastasizing to the limb bone in each experimental group, which was consistent with the clinical regularity and characteristics of skeletal metastases with lung cancer. hTus, the model has been established triumphantly. However, there were no statistical differences in the average time consumed and skeletal metastatic rate among the four experimental groups. Conclusion hTe disruption in the bone can be clearly detected by MicroCT, which is beneift to supervise the osseous metastasis. We successfully developed the nude mice bone metastasis model of lung adenocarcinoma, which will pave the way for exploring novel prevention and therapy strategies clinically. hTe four cell lines varied in invasion and migration abilities in vitro, but there was no statistical difference in the metastatic ability in vivo, and the reason need to be explored further in future.