CAJ | 학술논문

目的探讨高效液相色谱分析法在 HbE 病筛查中的价值。方法应用高效液相色谱分析仪对24779例样本进行血红蛋白（Hb）分析，HbA2≥11％的样本进行 HbE基因检测。结果24779例标本中检出129例HbA2≥11％的样本，含量为14．0％～75．2％（27．2％±9．4％）。经基因检测均确诊为 HbE病，包括 HbE杂合子、HbE/α-珠蛋白生成障碍性贫血、HbE/β-珠蛋白生成障碍性贫血、HbE/α-β-珠蛋白生成障碍性贫血和 HbE纯合子，其HbA2含量分别为（26．1±1．3）％、（18．2±1．8）％、（45．9±8．0）％、38．6％和（73．9±1．8）％。经 ROC曲线分析 HbA2筛查 HbE的临界值为11．7％。结论高效液相色谱不仅能有效地筛查 HbE病，还可以通过 HbA2含量区分HbE病突变类型及其复合珠蛋白生成障碍性贫血的类型，是筛查HbE病的有效方法。
목적탐토고효액상색보분석법재 HbE 병사사중적개치。방법응용고효액상색보분석의대24779례양본진행혈홍단백（Hb）분석，HbA2≥11％적양본진행 HbE기인검측。결과24779례표본중검출129례HbA2≥11％적양본，함량위14．0％～75．2％（27．2％±9．4％）。경기인검측균학진위 HbE병，포괄 HbE잡합자、HbE/α-주단백생성장애성빈혈、HbE/β-주단백생성장애성빈혈、HbE/α-β-주단백생성장애성빈혈화 HbE순합자，기HbA2함량분별위（26．1±1．3）％、（18．2±1．8）％、（45．9±8．0）％、38．6％화（73．9±1．8）％。경 ROC곡선분석 HbA2사사 HbE적림계치위11．7％。결론고효액상색보불부능유효지사사 HbE병，환가이통과 HbA2함량구분HbE병돌변류형급기복합주단백생성장애성빈혈적류형，시사사HbE병적유효방법。
Objective To explore the value of high performance liquid chromatography in the screening for HbE disease .Methods 24 779 samples were detected for hemoglobin by high performance liquid chromatography , and HbA2≥ 11% samples for genetic testing of HbE .Results Among 24 779 samples identifying 129 samples with HbA2≥11% ,the content was 14 .0% -75 .2% (27 .2% ± 9 .4% ) .Genetic testing were diagnosed as HbE disease ,in-cluding HbE heterozygous ,HbE/α-thalassemia ,HbE/β-thalassemia homozygotes ,HbE composited alpha and beta thalassemia and HbE homozygotes ,the HbA2 of which were (26 .1 ± 1 .3)% ,(18 .2 ± 1 .8)% ,(45 .9 ± 8 .0) ,38 .6%and (73 .9 ± 1 .8)% .The cutoff value of HbA2 in the screening for HbE disease was 11 .7% by ROC curve analysis . Conclusion High performance liquid chromatography is an effective screening method for HbE disease ,which can distinguish between HbE disease mutation type and composite thalassemia by HbA 2 content ,is an effective method for screening HbE disease .