CAJ | 학술논문

目的评估促凝剂对乙型肝炎病毒实时荧光定量检测的影响。方法随机采集2011年10月至2012年3月到西安市中心医院门诊的41例患者每人2管血样，分别为无添加剂普通管和促凝剂管，检测分离血清时间及效果。采用实时荧光定量聚合酶链反应检测乙型肝炎病毒DNA ，结果对数值采用SPSS11．5统计软件进行处理，并对促凝剂管标本进行精密度和抗干扰性能评价。结果促凝剂管分离血清平均时间为（4．73±0．86）min ，普通管为（14．46±2．27）min ，两组数据经配对 t检验，差异有统计学意义（P＜0．01）；促凝剂管乙型肝炎病毒DNA结果对数平均值为（3．86±2．99），普通管组对数平均值为（3．85±2．96），两组经配对 t检验，差异无统计学意义（P＞0．05），促凝剂管具有高精密度和抗干扰性能。结论促凝剂对乙型肝炎病毒实时荧光定量检测结果准确性没有影响，可减少报告周转时间，值得临床推广和应用。
목적평고촉응제대을형간염병독실시형광정량검측적영향。방법수궤채집2011년10월지2012년3월도서안시중심의원문진적41례환자매인2관혈양，분별위무첨가제보통관화촉응제관，검측분리혈청시간급효과。채용실시형광정량취합매련반응검측을형간염병독DNA ，결과대수치채용SPSS11．5통계연건진행처리，병대촉응제관표본진행정밀도화항간우성능평개。결과촉응제관분리혈청평균시간위（4．73±0．86）min ，보통관위（14．46±2．27）min ，량조수거경배대 t검험，차이유통계학의의（P＜0．01）；촉응제관을형간염병독DNA결과대수평균치위（3．86±2．99），보통관조대수평균치위（3．85±2．96），량조경배대 t검험，차이무통계학의의（P＞0．05），촉응제관구유고정밀도화항간우성능。결론촉응제대을형간염병독실시형광정량검측결과준학성몰유영향，가감소보고주전시간，치득림상추엄화응용。
Objective To evaluate effect of the coagulant on hepatitis B virus by real-time fluorescent quantita-tive detection .Methods From October 2011 to March 2012 were randomly collected in Xi'an city central hospital of 41 patients with each of 2 tubes of blood samples .which were without additives common tube and the coagulant tube . To detected the serum separation time and effect ,using real-time fluorescence quantitative PCR for detection of hepa-titis B virus DNA ,logarithmic mean was performed by SPSS11 .5 statistical analysis ,and the coagulant tube speci-mens for precision and anti-jamming performance evaluation .Results Coagulant group separation of serum average time was (4 .73 ± 0 .86)min ,common tube group was (14 .46 ± 2 .27)min ,data from the two groups were paired t test ,the difference was statistically significant (P<0 .01);Logarithmic mean in coagulant group of hepatitis B virus DNA was (3 .85 ± 2 .96) ,and that was (3 .86 ± 2 .99) in common tube group ,using paired t test ,the difference be-tween the two groups was not statistically significant (P>0 .05) .coagulant tube had the advantages of high precision and anti-jamming performance .Conclusion The coagulant on hepatitis B virus by real-time fluorescent quantitative detection accuracy is not affected ,can reduce the report turnaround time ,is worth the clinical promotion and applica-tion .