中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2014年
5期
616-619
,共4页
烟酰胺磷酸核糖基转移酶%脂肪肝%大鼠,Sprague-Dawley
煙酰胺燐痠覈糖基轉移酶%脂肪肝%大鼠,Sprague-Dawley
연선알린산핵당기전이매%지방간%대서,Sprague-Dawley
Nicotinamide phosphoribosyltransferase%Fatty liver%Rats,Sprague-Dawley
目的 观测内脂素(Visfatin)在非酒精性脂肪肝病大鼠肝组织中的表达情况,拟为非酒精性脂肪肝病的诊治开阔出新的思路.方法 将40只SD大鼠按随机数字表法分为对照组和造模组,每组20只.对照组予基础饲料喂养,并每周两次腹腔内注射生理盐水(0.2 ml/100 g体质量);造模组予高脂饲料喂养,并每周两次腹腔内注射40％ CCl4色拉油溶液(0.2 ml/100 g体质量).分析各组在2、4、6、8周4个时间点血清ALT、AST、TG、TC的变化及肝组织Visfatin的表达情况.结果 随着造模时间延长,造模组大鼠血清ALT、AST、TG、TC逐渐升高(P＜0.05).免疫组化及RT-PCR显示造模组大鼠各时期肝组织中内脂素水平随着造模时间的延长而增高,较同期对照组比较差异有统计学意义(P＜0.05).结论 (1)高脂喂养并腹腔内注射CCl4可以成功构建SD大鼠非酒精性脂肪肝病模型;(2)内脂素在非酒精性脂肪肝病大鼠肝脏组织中的表达水平随着肝脏脂肪变性程度增强而增高,因此可作为诊治非酒精性脂肪肝病并观测其严重程度的新指标.
目的 觀測內脂素(Visfatin)在非酒精性脂肪肝病大鼠肝組織中的錶達情況,擬為非酒精性脂肪肝病的診治開闊齣新的思路.方法 將40隻SD大鼠按隨機數字錶法分為對照組和造模組,每組20隻.對照組予基礎飼料餵養,併每週兩次腹腔內註射生理鹽水(0.2 ml/100 g體質量);造模組予高脂飼料餵養,併每週兩次腹腔內註射40％ CCl4色拉油溶液(0.2 ml/100 g體質量).分析各組在2、4、6、8週4箇時間點血清ALT、AST、TG、TC的變化及肝組織Visfatin的錶達情況.結果 隨著造模時間延長,造模組大鼠血清ALT、AST、TG、TC逐漸升高(P＜0.05).免疫組化及RT-PCR顯示造模組大鼠各時期肝組織中內脂素水平隨著造模時間的延長而增高,較同期對照組比較差異有統計學意義(P＜0.05).結論 (1)高脂餵養併腹腔內註射CCl4可以成功構建SD大鼠非酒精性脂肪肝病模型;(2)內脂素在非酒精性脂肪肝病大鼠肝髒組織中的錶達水平隨著肝髒脂肪變性程度增彊而增高,因此可作為診治非酒精性脂肪肝病併觀測其嚴重程度的新指標.
목적 관측내지소(Visfatin)재비주정성지방간병대서간조직중적표체정황,의위비주정성지방간병적진치개활출신적사로.방법 장40지SD대서안수궤수자표법분위대조조화조모조,매조20지.대조조여기출사료위양,병매주량차복강내주사생리염수(0.2 ml/100 g체질량);조모조여고지사료위양,병매주량차복강내주사40％ CCl4색랍유용액(0.2 ml/100 g체질량).분석각조재2、4、6、8주4개시간점혈청ALT、AST、TG、TC적변화급간조직Visfatin적표체정황.결과 수착조모시간연장,조모조대서혈청ALT、AST、TG、TC축점승고(P＜0.05).면역조화급RT-PCR현시조모조대서각시기간조직중내지소수평수착조모시간적연장이증고,교동기대조조비교차이유통계학의의(P＜0.05).결론 (1)고지위양병복강내주사CCl4가이성공구건SD대서비주정성지방간병모형;(2)내지소재비주정성지방간병대서간장조직중적표체수평수착간장지방변성정도증강이증고,인차가작위진치비주정성지방간병병관측기엄중정도적신지표.
Objective To investigate the expression of visfatin in the livers of rats suffering from non-alcoholic fatty liver disease (NAFLD) and to expect the development of new diagnosis and treatment for NAFLD.Methods A total of 40 Sprague-Dawley (SD) rats was randomly divided into control and NAFLD model groups.For control group,the rats were given basic diet and subcutaneous injection of saline(0.2 ml/100 g weight)twice a week.However,rats in the model group were given high fat diet and were given subcutaneous injection of 40 ％ carbon tetrachloride (CCl4) salad oil (0.2 ml/100 g) twice a week.The levels of alanine aminotransferase (ALT),aspartate aminotransferase (AST),triglyeride (TG),total-cholesterol (TC) in serum together with the visfatin expression in hepatic tissue were analyzed at the 2nd,4th,6th,and 8th weeks after operation,respectively.Results The levels of ALT,AST,TG,and TC in model group were increased gradually as time going(P ＜ 0.05).Immunohistochemistry and reverse transcription polymerase chain reaction(RT-PCR) experiments showed the level of visfatin in model group was significantly higher than control group in each period,with a increasing trends with the modeling time (P ＜ 0.05).Conclusions (1)After high-fat feeding and intraperitoneal injection of CCL4,a model of adult SD rats suffering from NAFLD was constructed.(2)In livers of rats suffering from NAFLD,the expression of visfatin was increased while the level of liver steatosis was enhanced.Visfatin would be a new target to observe the severity of NAFLD.
