中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2013年
5期
332-335
,共4页
房婕%李志豪%陈榕%姜培红
房婕%李誌豪%陳榕%薑培紅
방첩%리지호%진용%강배홍
尖锐湿疣%α乳头状瘤病毒属%Toll样受体7%T淋巴细胞%肿瘤坏死因子受体相关肽和相关蛋白质类%髓系分化因子88
尖銳濕疣%α乳頭狀瘤病毒屬%Toll樣受體7%T淋巴細胞%腫瘤壞死因子受體相關肽和相關蛋白質類%髓繫分化因子88
첨예습우%α유두상류병독속%Toll양수체7%T림파세포%종류배사인자수체상관태화상관단백질류%수계분화인자88
Condylomata acuminata%Alphapapillomavirus%Toll-like receptor 7%T-lymphocytes%Tumor necrosis factor receptor-associated peptides and proteins%MyD88
目的 探讨Toll样受体7(TLR7)及其相关信号转导分子在人类乳头瘤病毒(HPV)感染和尖锐湿疣复发机制中的作用.方法 用双色免疫荧光抗体染色流式细胞仪检测30例健康人、35例初发尖锐湿疣患者,32例复发尖锐湿疣患者和30例使用咪喹莫特联合氨基酮戊酸光动力疗法(ALA-PDT)治疗的复发性尖锐湿疣患者外周血不同亚群T细胞内TLR7的表达,使用SPSS 13.0软件进行t检验.用Western印迹方法检测各组尖锐湿疣患者及健康人外周血CD3+T细胞内接头蛋白MyD88及相关信号分子TRAF6、PI3K、AKT、p42/44和核因子κB(NF-κB)等表达的变化.结果 健康人外周血CD3+ CD4+T细胞和CD3+CD8+T细胞内均可检测到TLR7的表达.与健康对照组比较,初发和复发尖锐湿疣患者外周血CD3+CD8+T细胞内TLR7的表达水平无显著改变,而CD3+CD4+T细胞内TLR7的表达明显增加(健康对照组为12.6％±6.3％,初发尖锐湿疣组为23.3％±8.4％,t=4.72,P＜ 0.01;复发尖锐湿疣组为32.8％±8.9％,t=10.76,P＜0.01);与复发尖锐湿疣患者比较,咪喹莫特联合ALA-PDT治疗组尖锐湿疣患者外周血CD3+CD4+T细胞内TLR7的表达水平显著降低(20.3％±5.7％,t=5.41,P＜0.01).初发和复发尖锐湿疣患者外周血CD3+T细胞内MyD88、TRAF6、PI3K、p42/44和NF-κB蛋白表达显著增加,而AKT蛋白表达变化不明显;咪喹莫特联合ALA-PDT治疗组尖锐湿疣患者外周血CD3+T细胞内MyD88、TRAF6、p42/44和NF-κB蛋白表达显著降低.结论 尖锐湿疣患者外周血CD3+CD4+T细胞内表达上调的TLR7可作为HPV病毒感染的识别受体,参与机体的抗HPV免疫应答.
目的 探討Toll樣受體7(TLR7)及其相關信號轉導分子在人類乳頭瘤病毒(HPV)感染和尖銳濕疣複髮機製中的作用.方法 用雙色免疫熒光抗體染色流式細胞儀檢測30例健康人、35例初髮尖銳濕疣患者,32例複髮尖銳濕疣患者和30例使用咪喹莫特聯閤氨基酮戊痠光動力療法(ALA-PDT)治療的複髮性尖銳濕疣患者外週血不同亞群T細胞內TLR7的錶達,使用SPSS 13.0軟件進行t檢驗.用Western印跡方法檢測各組尖銳濕疣患者及健康人外週血CD3+T細胞內接頭蛋白MyD88及相關信號分子TRAF6、PI3K、AKT、p42/44和覈因子κB(NF-κB)等錶達的變化.結果 健康人外週血CD3+ CD4+T細胞和CD3+CD8+T細胞內均可檢測到TLR7的錶達.與健康對照組比較,初髮和複髮尖銳濕疣患者外週血CD3+CD8+T細胞內TLR7的錶達水平無顯著改變,而CD3+CD4+T細胞內TLR7的錶達明顯增加(健康對照組為12.6％±6.3％,初髮尖銳濕疣組為23.3％±8.4％,t=4.72,P＜ 0.01;複髮尖銳濕疣組為32.8％±8.9％,t=10.76,P＜0.01);與複髮尖銳濕疣患者比較,咪喹莫特聯閤ALA-PDT治療組尖銳濕疣患者外週血CD3+CD4+T細胞內TLR7的錶達水平顯著降低(20.3％±5.7％,t=5.41,P＜0.01).初髮和複髮尖銳濕疣患者外週血CD3+T細胞內MyD88、TRAF6、PI3K、p42/44和NF-κB蛋白錶達顯著增加,而AKT蛋白錶達變化不明顯;咪喹莫特聯閤ALA-PDT治療組尖銳濕疣患者外週血CD3+T細胞內MyD88、TRAF6、p42/44和NF-κB蛋白錶達顯著降低.結論 尖銳濕疣患者外週血CD3+CD4+T細胞內錶達上調的TLR7可作為HPV病毒感染的識彆受體,參與機體的抗HPV免疫應答.
목적 탐토Toll양수체7(TLR7)급기상관신호전도분자재인류유두류병독(HPV)감염화첨예습우복발궤제중적작용.방법 용쌍색면역형광항체염색류식세포의검측30례건강인、35례초발첨예습우환자,32례복발첨예습우환자화30례사용미규막특연합안기동무산광동력요법(ALA-PDT)치료적복발성첨예습우환자외주혈불동아군T세포내TLR7적표체,사용SPSS 13.0연건진행t검험.용Western인적방법검측각조첨예습우환자급건강인외주혈CD3+T세포내접두단백MyD88급상관신호분자TRAF6、PI3K、AKT、p42/44화핵인자κB(NF-κB)등표체적변화.결과 건강인외주혈CD3+ CD4+T세포화CD3+CD8+T세포내균가검측도TLR7적표체.여건강대조조비교,초발화복발첨예습우환자외주혈CD3+CD8+T세포내TLR7적표체수평무현저개변,이CD3+CD4+T세포내TLR7적표체명현증가(건강대조조위12.6％±6.3％,초발첨예습우조위23.3％±8.4％,t=4.72,P＜ 0.01;복발첨예습우조위32.8％±8.9％,t=10.76,P＜0.01);여복발첨예습우환자비교,미규막특연합ALA-PDT치료조첨예습우환자외주혈CD3+CD4+T세포내TLR7적표체수평현저강저(20.3％±5.7％,t=5.41,P＜0.01).초발화복발첨예습우환자외주혈CD3+T세포내MyD88、TRAF6、PI3K、p42/44화NF-κB단백표체현저증가,이AKT단백표체변화불명현;미규막특연합ALA-PDT치료조첨예습우환자외주혈CD3+T세포내MyD88、TRAF6、p42/44화NF-κB단백표체현저강저.결론 첨예습우환자외주혈CD3+CD4+T세포내표체상조적TLR7가작위HPV병독감염적식별수체,삼여궤체적항HPV면역응답.
Objective To explore the role of Toll-like receptor 7 (TLR7) and related signal transduction molecules in mechanisms underlying human papilloma virus (HPV) infection and condyloma acuminatum (CA) recurrence.Methods Peripheral blood was obtained from 30 healthy controls,35 patients with primary CA,32 patiens with recurrent CA and 30 patients with recurrent CA treated by imiquimod and ALA-PDT.Two-color flow cytometric analysis was used to detect TLR7 expression in different peripheral blood T cell subsets,Western blot to determine the expression levels of an adapter protein myeloid differentiation primary response gene 88 (MyD88),and signaling molecules including tumor necrosis factor receptor-associated factor (TRAF6),phosphatidylinositol3-kinase (PI3K),protein kinase B (AKT),p42/44 and nuclear factor-kappa B (NF-κB) in peripheral blood CD3+ T cells,from these subjects.Statistical analysis was carried out by Student's t test using the software SPSS 13.0.Results TLR7 was expressed in peripheral blood CD3+CD4+ T cells and CD3+CD8+ T cells from the healthy controls.Compared with the healthy controls,the patients with primary and recurrent CA showed no significant changes in TLR7 expression in CD3+CD8+ T cells,but a statistical increase in TLR7 expression in CD3+CD4+ T cells (23.3％ ± 8.4％ and 32.8％ ± 8.9％ vs.12.6％ ± 6.3％,t =4.72,10.76,both P ＜ 0.01).Decreased expression of TLR7 in CD3+CD4+ T cells was observed in patients treated with imiquimod and ALAPDT compared with the patients with recurrent CA (20.3％ ± 5.7％ vs.32.8％ ±8.9％,t =5.41,P ＜ 0.01).The protein expressions of MyD88,TRAF6,PI3K,p42/44 and NF-κB were significantly increased in CD3 + T cells,while the AKT protein expression experienced no significant changes in patients with primary or recurrent CA compared with the healthy controls.A significant decrease was observed in the protein expression of MyD88,TRAF6,p42/44 and NF-κB in the treated patients compared with those with recurrent CA.Conclusions TLR7,which is highly expressed in peripheral blood CD3+CD4+ T cells in patients with CA,may take part in the host immune response against HPV and serve as a recognition receptor for HPV infection.
