中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2013年
10期
749-752
,共4页
李洁清%侯殿俊%马娅%封丽%李卫国%刘伟%乔建维%贾喜明%李坤
李潔清%侯殿俊%馬婭%封麗%李衛國%劉偉%喬建維%賈喜明%李坤
리길청%후전준%마아%봉려%리위국%류위%교건유%가희명%리곤
电离辐射%基因芯片%基因表达谱%差异表达基因
電離輻射%基因芯片%基因錶達譜%差異錶達基因
전리복사%기인심편%기인표체보%차이표체기인
Ionizing radiation%Gene microarray%Gene expression profile%Differentially expressed gene
目的 应用基因芯片分析0.1 GyX射线照射对正常人淋巴细胞基因表达的影响,探讨低剂量辐射生物效应的作用机制.方法 采用含有45033个基因的NimbleGen 12×135K芯片分析0.1 GyX射线照射AHH-1细胞后培养6h和20 h基因表达谱的改变,照射组与对照组细胞的基因表达量差异比值大于2或小于0.5的基因确定为有效差异表达基因.用RT-PCR和real-time PCR方法验证部分差异表达基因.结果 0.1 Gy照射后6h,760个基因表达上调,1222个基因表达下调；照射后20 h,上调和下调基因分别是463和753个,共同差异表达的基因有92个.RT-PCR和real-time PCR方法验证了GADD45A、CDKN2A和Cx43的表达与基因芯片结果一致.结论 低剂量辐射对多种功能基因的表达产生了影响,可以为辐射损伤机制的研究提供依据.
目的 應用基因芯片分析0.1 GyX射線照射對正常人淋巴細胞基因錶達的影響,探討低劑量輻射生物效應的作用機製.方法 採用含有45033箇基因的NimbleGen 12×135K芯片分析0.1 GyX射線照射AHH-1細胞後培養6h和20 h基因錶達譜的改變,照射組與對照組細胞的基因錶達量差異比值大于2或小于0.5的基因確定為有效差異錶達基因.用RT-PCR和real-time PCR方法驗證部分差異錶達基因.結果 0.1 Gy照射後6h,760箇基因錶達上調,1222箇基因錶達下調；照射後20 h,上調和下調基因分彆是463和753箇,共同差異錶達的基因有92箇.RT-PCR和real-time PCR方法驗證瞭GADD45A、CDKN2A和Cx43的錶達與基因芯片結果一緻.結論 低劑量輻射對多種功能基因的錶達產生瞭影響,可以為輻射損傷機製的研究提供依據.
목적 응용기인심편분석0.1 GyX사선조사대정상인림파세포기인표체적영향,탐토저제량복사생물효응적작용궤제.방법 채용함유45033개기인적NimbleGen 12×135K심편분석0.1 GyX사선조사AHH-1세포후배양6h화20 h기인표체보적개변,조사조여대조조세포적기인표체량차이비치대우2혹소우0.5적기인학정위유효차이표체기인.용RT-PCR화real-time PCR방법험증부분차이표체기인.결과 0.1 Gy조사후6h,760개기인표체상조,1222개기인표체하조；조사후20 h,상조화하조기인분별시463화753개,공동차이표체적기인유92개.RT-PCR화real-time PCR방법험증료GADD45A、CDKN2A화Cx43적표체여기인심편결과일치.결론 저제량복사대다충공능기인적표체산생료영향,가이위복사손상궤제적연구제공의거.
Objective To investigate the effect of 0.1 Gy X-ray irradiation on the gene expression profiles in normal human lymphoblastoid cells using gene microarray and to explore the possible mechanism of the biological effect of low-dose irradiation.Methods The NimbleGen 12 ×135 K microarray corresponding to 45033 genes was used to analyze the gene expression profiles in AHH-1 cells cultured for 6 h and 20 h after 0.1 Gy X-ray irradiation.A gene was identified as the differentially expressed gene if the ratio between its expression levels in irradiation group and control group was higher than 2 or lower than 0.5.RT-PCR and real-time PCR were used to confirm some differentially expressed genes.Results There were 760 up-regulated genes and 1222 down-regulated genes in the cells at 6 h after 0.1 Gy X-ray irradiation,while there were 463 up-regulated genes and 753 down-regulated genes at 20 h after 0.1 Gy X-ray irradiation; there were 92 differentially expressed genes in common.The expression of GADD45A,CDKN2A,and Cx43 measured using gene microarray was confirmed by RT-PCR and real-time PCR.Conclusion Low-dose irradiation can affect the expression of many functional genes,which provides a basis for the research on the mechanism of radiation damage.
