CAJ | 학술논문

背景：研究者们正尝试应用各种方法研究肝癌的发生机制、干预措施等，间充质干细胞因其具有低免疫原性及趋瘤性等特点，成为肿瘤研究热门的工具细胞。目前间充质干细胞已经开始应用于肝癌的研究中。 　　目的：综述近年来间充质干细胞运用于肝癌基础与临床的研究进展。 　　方法：第一作者应用计算机检索2004年1月至2013年1月PubMed数据库、中国期刊全文数据库有关间充质干细胞、经修饰的间充质干细胞对肝癌细胞影响效应及其作用机制的文章，英文检索词“mesenchymal stem cel ，liver cancer，tumor”，中文检索词“间充质干细胞，肝癌，肿瘤”。排除重复性研究，47篇文献符合纳入标准。 　　结果与结论：干细胞运用于肝癌的研究报道较少，目前的研究结果显示，间充质干细胞对肝癌细胞增殖、侵袭等生物学行为有一定影响，然而因各实验室使用的细胞株种类、实验条件、动物模型、干细胞输注途径等诸多因素的不同，实验结果也不一致。学者们主要针对Wnt途径、肿瘤坏死因子相关凋亡诱导配体途径等机制进行了一系列的研究。间充质干细胞对包括肝癌在内的肿瘤细胞具有趋向性是公认的，利用这一特性，已经有学者将治疗基因、药物等导入或修饰间充质干细胞用于干预肝癌的发展，并取得了进展，为肝癌的细胞治疗提供了新的途径。但目前对间充质干细胞的体内安全性研究及临床实验报道较少，存在安全隐患，需要进一步研究探索。
배경：연구자문정상시응용각충방법연구간암적발생궤제、간예조시등，간충질간세포인기구유저면역원성급추류성등특점，성위종류연구열문적공구세포。목전간충질간세포이경개시응용우간암적연구중。 　　목적：종술근년래간충질간세포운용우간암기출여림상적연구진전。 　　방법：제일작자응용계산궤검색2004년1월지2013년1월PubMed수거고、중국기간전문수거고유관간충질간세포、경수식적간충질간세포대간암세포영향효응급기작용궤제적문장，영문검색사“mesenchymal stem cel ，liver cancer，tumor”，중문검색사“간충질간세포，간암，종류”。배제중복성연구，47편문헌부합납입표준。 　　결과여결론：간세포운용우간암적연구보도교소，목전적연구결과현시，간충질간세포대간암세포증식、침습등생물학행위유일정영향，연이인각실험실사용적세포주충류、실험조건、동물모형、간세포수주도경등제다인소적불동，실험결과야불일치。학자문주요침대Wnt도경、종류배사인자상관조망유도배체도경등궤제진행료일계렬적연구。간충질간세포대포괄간암재내적종류세포구유추향성시공인적，이용저일특성，이경유학자장치료기인、약물등도입혹수식간충질간세포용우간예간암적발전，병취득료진전，위간암적세포치료제공료신적도경。단목전대간충질간세포적체내안전성연구급림상실험보도교소，존재안전은환，수요진일보연구탐색。
BACKGROUND:Liver cancer pathogenesis and intervention have attracted increasing attentions. Mesenchymal stem cel s become a popular tool for cel cancer research, because of their low immunogenicity and tumor tropism. At present, mesenchymal stem cel s have been applied to the study of liver cancer. OBJECTIVE:To summarize the advances of mesenchymal stem cel s used in liver cancer in basic and clinical research. METHODS:An online retrieval of CNKI and Pubmed database was performed by the first author for the articles about mesenchymal stem cel s and effect of modified mesenchymal stem cel s on hepatoma carcinoma cel s published from January 2004 to January 2013. The key words were“mesenchymal stem cel , liver cancer, tumor”in Chinese and English. Repetitive research was excluded, and 47 studies met the inclusion criteria. RESULTS AND CONCLUSION:Stem cel s are seldom reported in liver cancer, and the limited present study show that mesenchymal stem cel s may have a certain influence on the hepatoma carcinoma cel proliferation, invasion and biological behavior. However, due to the differences of cel lines used by the various laboratories, experimental conditions, animal models, as wel as infusion means of stem cel s, experimental results are also inconsistent. Scholars have conducted a series of studies on the mechanism of the Wnt pathway and tumor necrosis factor-related apoptosis-inducing ligand pathway. Tropism of mesenchymal stem cel s to tumor cel s, including liver cancer is widely recognized, so scholars imported therapeutic genes and drugs into mesenchymal stem cel s to interfere with the development of liver cancer, and have achieved some progress. This evidence provides new avenues for cel therapy for liver cancer. Less safety studies in vivo and clinical trials of mesenchymal stem cel s are available, therefore security risks deserve further research.