中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2013年
36期
6515-6520
,共6页
宋雪云%龙云%谭超%郑景辉
宋雪雲%龍雲%譚超%鄭景輝
송설운%룡운%담초%정경휘
干细胞%干细胞与中医药%丹参酮Ⅱa%黄芪甲苷%骨髓间充质干细胞%心肌样细胞%诱导分化%有效部位%心肌缺血%国家自然科学基金
榦細胞%榦細胞與中醫藥%丹參酮Ⅱa%黃芪甲苷%骨髓間充質榦細胞%心肌樣細胞%誘導分化%有效部位%心肌缺血%國傢自然科學基金
간세포%간세포여중의약%단삼동Ⅱa%황기갑감%골수간충질간세포%심기양세포%유도분화%유효부위%심기결혈%국가자연과학기금
背景：黄芪甲苷和丹参酮Ⅱa 是传统中医药治疗心肌缺血的有效成分，是否可参与骨髓间充质干细胞向心肌样细胞分化的过程呢？<br> 目的：观察黄芪甲苷联合丹参酮Ⅱa对体外诱导骨髓间充质干细胞向心肌样细胞分化作用的影响。<br> 方法：应用MTT法分别测定黄芪甲苷和丹参酮Ⅱa的最大无毒浓度，确定两者联合诱导骨髓间充质干细胞分化为心肌样细胞的剂量。将分离纯化的骨髓间充质干细胞分5组进行诱导：黄芪甲苷组、丹参酮Ⅱa 组、丹参酮Ⅱa+黄芪甲苷组、5-氮胞苷组、空白对照组，ELISA方法观察体外诱导后骨髓间充质干细胞缝隙连接蛋白43和肌钙蛋白表达变化。<br> 结果与结论：诱导后丹参酮Ⅱa+黄芪甲苷组、丹参酮Ⅱa、黄芪甲苷、5-氮胞苷组肌钙蛋白、缝隙连接蛋白43表达水平均高于正常对照组(P <0.01)，丹参酮Ⅱa+黄芪甲苷组高于丹参酮Ⅱa、黄芪甲苷组(P <0.01)。丹参酮Ⅱa+黄芪甲苷组与5-氮胞苷组缝隙连接蛋白43表达水平差异无显著性意义(P>0.05)。结果提示黄芪甲苷联合丹参酮Ⅱa可以诱导骨髓间充质干细胞向心肌样细胞方向转化，而其联合作用高于单一成分的作用。
揹景：黃芪甲苷和丹參酮Ⅱa 是傳統中醫藥治療心肌缺血的有效成分，是否可參與骨髓間充質榦細胞嚮心肌樣細胞分化的過程呢？<br> 目的：觀察黃芪甲苷聯閤丹參酮Ⅱa對體外誘導骨髓間充質榦細胞嚮心肌樣細胞分化作用的影響。<br> 方法：應用MTT法分彆測定黃芪甲苷和丹參酮Ⅱa的最大無毒濃度，確定兩者聯閤誘導骨髓間充質榦細胞分化為心肌樣細胞的劑量。將分離純化的骨髓間充質榦細胞分5組進行誘導：黃芪甲苷組、丹參酮Ⅱa 組、丹參酮Ⅱa+黃芪甲苷組、5-氮胞苷組、空白對照組，ELISA方法觀察體外誘導後骨髓間充質榦細胞縫隙連接蛋白43和肌鈣蛋白錶達變化。<br> 結果與結論：誘導後丹參酮Ⅱa+黃芪甲苷組、丹參酮Ⅱa、黃芪甲苷、5-氮胞苷組肌鈣蛋白、縫隙連接蛋白43錶達水平均高于正常對照組(P <0.01)，丹參酮Ⅱa+黃芪甲苷組高于丹參酮Ⅱa、黃芪甲苷組(P <0.01)。丹參酮Ⅱa+黃芪甲苷組與5-氮胞苷組縫隙連接蛋白43錶達水平差異無顯著性意義(P>0.05)。結果提示黃芪甲苷聯閤丹參酮Ⅱa可以誘導骨髓間充質榦細胞嚮心肌樣細胞方嚮轉化，而其聯閤作用高于單一成分的作用。
배경：황기갑감화단삼동Ⅱa 시전통중의약치료심기결혈적유효성분，시부가삼여골수간충질간세포향심기양세포분화적과정니？<br> 목적：관찰황기갑감연합단삼동Ⅱa대체외유도골수간충질간세포향심기양세포분화작용적영향。<br> 방법：응용MTT법분별측정황기갑감화단삼동Ⅱa적최대무독농도，학정량자연합유도골수간충질간세포분화위심기양세포적제량。장분리순화적골수간충질간세포분5조진행유도：황기갑감조、단삼동Ⅱa 조、단삼동Ⅱa+황기갑감조、5-담포감조、공백대조조，ELISA방법관찰체외유도후골수간충질간세포봉극련접단백43화기개단백표체변화。<br> 결과여결론：유도후단삼동Ⅱa+황기갑감조、단삼동Ⅱa、황기갑감、5-담포감조기개단백、봉극련접단백43표체수평균고우정상대조조(P <0.01)，단삼동Ⅱa+황기갑감조고우단삼동Ⅱa、황기갑감조(P <0.01)。단삼동Ⅱa+황기갑감조여5-담포감조봉극련접단백43표체수평차이무현저성의의(P>0.05)。결과제시황기갑감연합단삼동Ⅱa가이유도골수간충질간세포향심기양세포방향전화，이기연합작용고우단일성분적작용。
BACKGROUND:Astragaloside and tanshinone IIa are the effective components of traditional Chinese medicine treatment of myocardial ischemia, and its role in bone marrow mesenchymal stem cel s differentiation into myocardium-like cel s remains unclear. <br> OBJECTIVE:To investigate the effect of tanshinone IIa and astragaloside on the differentiation of bone marrow mesenchymal stem cel s into myocardium-like cel s. <br> METHODS:The maximal non-toxic concentrations of tanshinone IIa and astragaloside were measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, to define the dose of the two in the induced differentiation of bone marrow mesenchymal stem cel s into myocardium-like cel s. The isolated and purified bone marrow mesenchymal stem cel s were divided into five groups:astragaloside group, tanshinone IIa group, astragaloside+tanshinone IIa group, 5-azacitidine group, and blank control group. The expression of gap junction connexin 43 and troponin was determined with enzyme-linked immunosorbent assay. <br> RESULTS AND CONCLUSION:The expression of gap junction connexin 43 and troponin in astragaloside group, tanshinone IIa group, astragaloside+tanshinone IIa group, 5-azacitidine group was higher than that in blank control group (P<0.01). The astragaloside+tanshinone IIa group showed a higher expression of gap junction connexin 43 and troponin than astragaloside group and tanshinone IIa group (P<0.01). There was no significant difference in the expression of gap junction connexin 43 and troponin between astragaloside+tanshinone IIa group and 5-azacitidine group (P>0.05). A combined use of astragaloside and tanshinone IIa can induce bone marrow mesenchymal stem cel s to differentiate into myocardium-like cel s, and their joint role is better than the role of a single ingredient.
