中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2013年
36期
6501-6507
,共7页
荣春%申延清%吕亚青%李明玉%夏长所
榮春%申延清%呂亞青%李明玉%夏長所
영춘%신연청%려아청%리명옥%하장소
干细胞%干细胞培养与分化%富血小板血浆%间充质干细胞%增殖%Ⅰ型胶原%Ⅲ型胶原%细胞培养%细胞分化%肌腱细胞
榦細胞%榦細胞培養與分化%富血小闆血漿%間充質榦細胞%增殖%Ⅰ型膠原%Ⅲ型膠原%細胞培養%細胞分化%肌腱細胞
간세포%간세포배양여분화%부혈소판혈장%간충질간세포%증식%Ⅰ형효원%Ⅲ형효원%세포배양%세포분화%기건세포
背景：间充质干细胞是可大量获取的肌腱组织工程种子细胞，但如何体外诱导成为此项技术的关键。<br> 目的：观察富血小板血浆对体外培养间充质干细胞分裂增殖和胶原产生的影响。<br> 方法：分离培养兔间充质干细胞，设立富血小板血浆高、中、低剂量组干预间充质干细胞，并设空白组做对照。<br> 结果与结论：富血小板血浆高、中、低组间充质干细胞均保持较高的增殖活性，呈快速生长，曲线上升幅度大，与空白对照组比较差异有显著性意义(P <0.05)。培养时间越长作用越明显，培养一定时间后其作用具有剂量依赖性，较高剂量的富血小板血浆对细胞的增殖作用较为明显。提示富血小板血浆能明显促进间充质干细胞的Ⅰ和Ⅲ型胶原合成，剂量越大，刺激胶原产生的作用越明显。
揹景：間充質榦細胞是可大量穫取的肌腱組織工程種子細胞，但如何體外誘導成為此項技術的關鍵。<br> 目的：觀察富血小闆血漿對體外培養間充質榦細胞分裂增殖和膠原產生的影響。<br> 方法：分離培養兔間充質榦細胞，設立富血小闆血漿高、中、低劑量組榦預間充質榦細胞，併設空白組做對照。<br> 結果與結論：富血小闆血漿高、中、低組間充質榦細胞均保持較高的增殖活性，呈快速生長，麯線上升幅度大，與空白對照組比較差異有顯著性意義(P <0.05)。培養時間越長作用越明顯，培養一定時間後其作用具有劑量依賴性，較高劑量的富血小闆血漿對細胞的增殖作用較為明顯。提示富血小闆血漿能明顯促進間充質榦細胞的Ⅰ和Ⅲ型膠原閤成，劑量越大，刺激膠原產生的作用越明顯。
배경：간충질간세포시가대량획취적기건조직공정충자세포，단여하체외유도성위차항기술적관건。<br> 목적：관찰부혈소판혈장대체외배양간충질간세포분렬증식화효원산생적영향。<br> 방법：분리배양토간충질간세포，설립부혈소판혈장고、중、저제량조간예간충질간세포，병설공백조주대조。<br> 결과여결론：부혈소판혈장고、중、저조간충질간세포균보지교고적증식활성，정쾌속생장，곡선상승폭도대，여공백대조조비교차이유현저성의의(P <0.05)。배양시간월장작용월명현，배양일정시간후기작용구유제량의뢰성，교고제량적부혈소판혈장대세포적증식작용교위명현。제시부혈소판혈장능명현촉진간충질간세포적Ⅰ화Ⅲ형효원합성，제량월대，자격효원산생적작용월명현。
BACKGROUND:Mesenchymal stem cel s are the seed cel s for tendon tissue engineering which can be obtained in large quantities, but how to induce in vitro is a key technology. <br> OBJECTIVE:To explore the effect of platelet-rich plasma on the proliferation and col agen production of in vitro cultured mesenchymal stem cel s. <br> METHODS:The rabbit mesenchymal stem cel s were separated ad cultured. The high-dose platelet-rich plasma group, middle-dose platelet-rich plasma group and low-dose platelet-rich plasma group were set to induce the mesenchymal stem cel s, and the blank control group was set as control. <br> RESULTS AND CONCLUSION:The proliferation of mesenchymal stem cel s in the high-dose platelet-rich plasma group, middle-dose platelet-rich plasma group and low-dose platelet-rich plasma group was high, and in rapid growth with big increase amplitude, and there was no significant difference in the proliferation when compared with the blank control group (P<0.05). The effect was positively correlated with the culture time, and after cultured for a certain time, the effect was in dose-dependent manner, as higher dose platelet-rich plasma had more significant effect on the proliferation of the cel s. The results indicate that platelet-rich plasma can significantly promote the synthesis of col agen type Ⅰ and Ⅲ of mesenchymal stem cel s, the higher the dose, the more significant the effect on the col agen.
