CAJ | 학술논문

背景：胚胎干细胞具有多向分化潜能，已被用于急性肝损伤的治疗，但其在体内治疗过程中的增殖迁移情况尚不清楚。 　　目的：利用分子影像技术监测移植胚胎干细胞在急性肝损伤修复过程中的行为。 　　方法：利用慢病毒载体，将萤火虫荧光素酶、红色荧光蛋白以及单纯疱疹病毒胸苷激酶三融合基因转入小鼠胚胎干细胞(D3)中，筛选得到稳定整合3个报告基因的D3胚胎干细胞系。将上述胚胎干细胞或分化了6 d的拟胚体细胞通过脾脏注射到急性肝损伤SV129模型小鼠体内，利用生物发光成像技术监测移植的细胞。 　　结果与结论：RT-PCR结果显示，三融合基因的转入并未影响胚胎干细胞Oct-4和Nanog的表达。利用小动物活体成像系统可以观察到移植细胞从脾脏迁移到肝脏的过程。移植的胚胎干细胞和拟胚体细胞都在肝脏处形成畸胎瘤，由于单纯疱疹病毒胸苷激酶同时也是自杀基因，可以与更昔洛韦作用诱导转基因细胞死亡，通过注射更昔洛韦来抑制畸胎瘤的生长并逐步将其杀死。组织学分析显示，畸胎瘤里包含来自于3个胚层的组织。提示三融合基因的转入并未影响胚胎干细胞的多向分化潜能，且胚胎干细胞用于细胞治疗有潜在的成瘤风险，影响了对肝损伤的修复，治疗策略有待进一步改进，并需要实时监控其在体内的行为。
배경：배태간세포구유다향분화잠능，이피용우급성간손상적치료，단기재체내치료과정중적증식천이정황상불청초。 　　목적：이용분자영상기술감측이식배태간세포재급성간손상수복과정중적행위。 　　방법：이용만병독재체，장형화충형광소매、홍색형광단백이급단순포진병독흉감격매삼융합기인전입소서배태간세포(D3)중，사선득도은정정합3개보고기인적D3배태간세포계。장상술배태간세포혹분화료6 d적의배체세포통과비장주사도급성간손상SV129모형소서체내，이용생물발광성상기술감측이식적세포。 　　결과여결론：RT-PCR결과현시，삼융합기인적전입병미영향배태간세포Oct-4화Nanog적표체。이용소동물활체성상계통가이관찰도이식세포종비장천이도간장적과정。이식적배태간세포화의배체세포도재간장처형성기태류，유우단순포진병독흉감격매동시야시자살기인，가이여경석락위작용유도전기인세포사망，통과주사경석락위래억제기태류적생장병축보장기살사。조직학분석현시，기태류리포함래자우3개배층적조직。제시삼융합기인적전입병미영향배태간세포적다향분화잠능，차배태간세포용우세포치료유잠재적성류풍험，영향료대간손상적수복，치료책략유대진일보개진，병수요실시감공기재체내적행위。
BACKGROUND:Embryonic stem cel s have the capacity of multi-differentiation potential, and have been utilized for the therapy of acute liver injury. However, the migration and proliferation of embryonic stem cel s after transplantation remains not wel characterized. OBJECTIVE:To track the transplanted embryonic stem cel s in repairing acute liver injury by bioluminescence imaging technology. METHODS:Murine embryonic stem cel s (D3) were transducted with a construct composed of firefly luciferase, monomeric red fluorescence protein and herpes simplex virus truncated thymidine kinase triple fusion reporter genes by lentivirus system. Stable D3 embryonic stem cel s integrating three report genes were screened. The undifferentiated embryonic stem cel s or differentiated embryonic stem cel s from the 6-day-old embryoid body were transplanted into acute liver injury model of SV129 mouse through spleen, and the transplanted cel s were monitored by bioluminescence imaging technology. RESULTS AND CONCLUSION:Reverse transcription PCR results showed that the expression level of Oct-4 and Nanog was not affected in embryonic stem cel s transducted with triple fusion reporter gene compared with wild-type embryonic stem cel s. The migration process of transplanted cel s was visualized by bioluminescence imaging technology. Teratomas were found in both triple fusion-embryonic stem cel s treatment group and triple fusion-embryoid body cel s treatment group at liver, and the teratoma formation could be suppressed by ganciclovir administration because ganciclovir can react with herpes simplex virus truncated thymidine kinase and trigger cel necrosis process. Histological analysis showed that teratomas comprised tissues from al three germ layers. These results demonstrate that triple gene fusion does not affect differentiation potential of embryonic stem cel s and it is risky to utilize embryonic stem cel s for cel therapy, because it affects repair of liver injury. The therapy strategy requires further improvement and real-time visualizing of embryonic stem cel s in vivo is absolutely necessary.