CAJ | 학술논문

背景：无血清培养对大鼠脂肪干细胞向血管内皮细胞诱导分化影响的报道甚少。 　　目的：观察无血清培养大鼠脂肪干细胞后向血管内皮细胞诱导分化的情况。 　　方法：采用酶消贴壁培养法获得雄性SD大鼠脂肪干细胞，传代培养至第3代。实验组细胞无血清培养24 h，对照组用含体积分数10%胎牛血清的L-DMEM完全培养基培养。然后应用血管内皮细胞诱导培养基培养3周。 　　结果与结论：大鼠脂肪干细胞经体外培养可呈多角形或梭形贴壁生长，并能稳定传代。传代后大鼠脂肪干细胞极低表达细胞表面标志CD31。大鼠脂肪干细胞定向血管内皮细胞诱导分化后细胞呈鹅卵石样，CD31阳性表达明显上升且实验组明显高于对照组。实验组诱导后大鼠脂肪干细胞能够吞噬Dil标记的乙酰化低密度脂蛋白及在基质胶上形成2D小管样结构，其能力明显强于对照组。结果证实无血清培养可促进体外大鼠脂肪干细胞向血管内皮细胞诱导分化。
배경：무혈청배양대대서지방간세포향혈관내피세포유도분화영향적보도심소。 　　목적：관찰무혈청배양대서지방간세포후향혈관내피세포유도분화적정황。 　　방법：채용매소첩벽배양법획득웅성SD대서지방간세포，전대배양지제3대。실험조세포무혈청배양24 h，대조조용함체적분수10%태우혈청적L-DMEM완전배양기배양。연후응용혈관내피세포유도배양기배양3주。 　　결과여결론：대서지방간세포경체외배양가정다각형혹사형첩벽생장，병능은정전대。전대후대서지방간세포겁저표체세포표면표지CD31。대서지방간세포정향혈관내피세포유도분화후세포정아란석양，CD31양성표체명현상승차실험조명현고우대조조。실험조유도후대서지방간세포능구탄서Dil표기적을선화저밀도지단백급재기질효상형성2D소관양결구，기능력명현강우대조조。결과증실무혈청배양가촉진체외대서지방간세포향혈관내피세포유도분화。
BACKGROUND:There are few reports about the effect of serum-free culture on the differentiation of rat adipose-derived stem cel s into vascular endothelial cel s. OBJECTIVE:To investigate the isolation, serum-free culture of rat adipose-derived stem cel s differentiating into vascular endothelial cel s. METHODS:The rat adipose-derived stem cel s were isolated from male Sprague-Dawley rats and expanded to the third passage by enzymatic digestion-adherent explants method. In the experimental group, rat adipose-derived stem cel s were cultured in serum-free medium for 24 hours. In the control group, rat adipose-derived stem cel s were cultured in low-glucose Dulbecco’s modified Eagle’s medium containing 10%fetal bovine serum. After that, the cel s were cultured in inducing medium for 3 weeks to differentiate into vascular endothelial cel s. RESULTS AND CONCLUSION:The rat adipose-derived stem cel s grew as polygonal or fusiform-shaped adherent cel s when cultured in vitro, which could stably proliferate and passage. The rat adipose-derived stem cel s showed very low expression of CD31, a cel surface marker, after passages. After directional differentiations into vascular endothelial cel s, the cel s were pebble-shaped under the inverted microscope. Expression of CD31 was up-regulated, which was much higher in the experimental group than the control group. The induced cel s in the experimental group had stronger abilities than those in the control group to swal ow Dil-labeled acetylated low-density lipoprotein and form tube-like structures on the matrigel after differentiation into vascular endothelial cel s. So, rat adipose-derived stem cel s could be highly successful y induced to differentiate into vascular endothelial cel s in vitro after serum-free culture.