CAJ | 학술논문

背景：国内外尚无对脐带间充质干细胞运输过程中相关活力指标的全面评估。 　　目的：检测白蛋白和储运时间等不同条件对脐带间充质干细胞存活率的影响。 　　方法：体外培养的脐带间充质干细胞分为实验组和对照组，每组将3.15×109 L-1细胞悬浮在3 mL生理盐水中。其中实验组加入1%白蛋白并避光保存，对照组分为不加白蛋白避光保存组和加白蛋白光照保存组，在0，2，4，8和24 h分别测定各组细胞数、锥虫蓝拒染率和细胞存活率。 　　结果与结论：与对照组相比，实验组(保存液中加白蛋白且避光)的8h细胞损失率几乎为零，细胞存活率接近90%。对照中不加白蛋白组8 h细胞损失率高达38.5%，细胞存活率为86%。在脐带间充质干细胞的长途运输中，加入1%白蛋白可以有效保证细胞存活量。运输时间超出8h后，细胞损失率上升且存活率下调。实验数据表明，16℃、避光和1%白蛋白生理盐水的保存条件下，脐带间充质干细胞在8 h内符合临床输注要求。
배경：국내외상무대제대간충질간세포운수과정중상관활력지표적전면평고。 　　목적：검측백단백화저운시간등불동조건대제대간충질간세포존활솔적영향。 　　방법：체외배양적제대간충질간세포분위실험조화대조조，매조장3.15×109 L-1세포현부재3 mL생리염수중。기중실험조가입1%백단백병피광보존，대조조분위불가백단백피광보존조화가백단백광조보존조，재0，2，4，8화24 h분별측정각조세포수、추충람거염솔화세포존활솔。 　　결과여결론：여대조조상비，실험조(보존액중가백단백차피광)적8h세포손실솔궤호위령，세포존활솔접근90%。대조중불가백단백조8 h세포손실솔고체38.5%，세포존활솔위86%。재제대간충질간세포적장도운수중，가입1%백단백가이유효보증세포존활량。운수시간초출8h후，세포손실솔상승차존활솔하조。실험수거표명，16℃、피광화1%백단백생리염수적보존조건하，제대간충질간세포재8 h내부합림상수주요구。
BACKGROUND:General assessment of viability of umbilical cord mesenchymal stem cel s during transport is not considered at home and abroad. OBJECTIVE:To explore the effects of different transportation conditions such as albumin and transport time on survival rate of umbilical cord mesenchymal stem cel s. METHODS:Umbilical cord mesenchymal stem cel s cultured in vitro were divided into experimental and control groups. Each of group contained 3.15×109/L cel s in 3 mL normal saline. The experimental group contained 1%albumin in dark environment. The control group included two subgroups:dark preservation group with the absence of albumin and light preservation group with the presence of albumin. Cel counting, trypan blue staining and cel survival rate were compared at 0, 2, 4, 8, and 24 hours. RESULTS AND CONCLUSION:Compared with the control group, no cel loss was observed in experimental group (with the presence of albumin in the dark) and a 90%viability ratio was achieved at 8 hours. In the control group without albumin, loss rate reached 38.5%and survival rate reached 86%at 8 hours. Results revealed that 1%albumin predominantly improved cel survival rate in long-distance transport of umbilical cord mesenchymal stem cel s. When transport time was more than 8 hours, cel loss rate increased and cel survival rate downregulated. Our experimental data demonstrated that umbilical cord mesenchymal stem cel s preserved in normal saline consisting of 1%albumin placing in dark environment at 16 ℃ apply to clinical application criterion in 8 hours.